Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22029 | 3' | -57.3 | NC_004927.1 | + | 63778 | 0.66 | 0.717203 |
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Target: 5'- gGUCGCCGUga-CACCcaCCUCGAUg- -3' miRNA: 3'- gCGGCGGCAaagGUGGa-GGGGCUAac -5' |
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| 22029 | 3' | -57.3 | NC_004927.1 | + | 3714 | 0.66 | 0.70691 |
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Target: 5'- gGCCGCUccg-CgACCUUCCCGAa-- -3' miRNA: 3'- gCGGCGGcaaaGgUGGAGGGGCUaac -5' |
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| 22029 | 3' | -57.3 | NC_004927.1 | + | 37799 | 0.66 | 0.692388 |
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Target: 5'- uGCCGCCGUcaugCCACacccaaugucgcCCCCGAc-- -3' miRNA: 3'- gCGGCGGCAaa--GGUGga----------GGGGCUaac -5' |
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| 22029 | 3' | -57.3 | NC_004927.1 | + | 30873 | 0.68 | 0.591347 |
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Target: 5'- -aUCGCC-UUUCCACacaUCCCCGaAUUGa -3' miRNA: 3'- gcGGCGGcAAAGGUGg--AGGGGC-UAAC- -5' |
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| 22029 | 3' | -57.3 | NC_004927.1 | + | 935 | 0.72 | 0.34569 |
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Target: 5'- cCGCC-CCGgUUCCGUCUCCCCGGUc- -3' miRNA: 3'- -GCGGcGGCaAAGGUGGAGGGGCUAac -5' |
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| 22029 | 3' | -57.3 | NC_004927.1 | + | 64259 | 0.74 | 0.286176 |
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Target: 5'- aGCCGCuaCGggaaUCCgACCUCCCCGAUg- -3' miRNA: 3'- gCGGCG--GCaa--AGG-UGGAGGGGCUAac -5' |
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| 22029 | 3' | -57.3 | NC_004927.1 | + | 9096 | 1.09 | 0.001112 |
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Target: 5'- gCGCCGCCGUUUCCACCUCCCCGAUUGa -3' miRNA: 3'- -GCGGCGGCAAAGGUGGAGGGGCUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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