Results 1 - 20 of 77 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22942 | 5' | -46.9 | NC_005137.2 | + | 99700 | 0.66 | 0.999789 |
Target: 5'- aACAAGCga-----GCGCGCuGACGCa -3' miRNA: 3'- -UGUUUGaguauuaCGCGCGuUUGCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 11389 | 0.66 | 0.999789 |
Target: 5'- aGCaAGugUUuu-GUGCGCGCAGaaaaaacuguauACGCGa -3' miRNA: 3'- -UG-UUugAGuauUACGCGCGUU------------UGCGC- -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 74736 | 0.66 | 0.999789 |
Target: 5'- cGCAGuccaugGC-CGUuuUGUGCGUAAACGCc -3' miRNA: 3'- -UGUU------UGaGUAuuACGCGCGUUUGCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 39522 | 0.66 | 0.999789 |
Target: 5'- uCAAACUCAacaucgauUGUGCGCAAcCGUu -3' miRNA: 3'- uGUUUGAGUauu-----ACGCGCGUUuGCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 15413 | 0.66 | 0.999789 |
Target: 5'- uUAAAUgagUAUGAUGCGCGUggAGugGCc -3' miRNA: 3'- uGUUUGa--GUAUUACGCGCG--UUugCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 110712 | 0.66 | 0.999729 |
Target: 5'- cGCGuGCgCAacgguGUGCGCGCAGAgCGCu -3' miRNA: 3'- -UGUuUGaGUau---UACGCGCGUUU-GCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 56728 | 0.66 | 0.999729 |
Target: 5'- cGCGccuCUUAUcAUGCGCG-AAACGCa -3' miRNA: 3'- -UGUuu-GAGUAuUACGCGCgUUUGCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 42359 | 0.66 | 0.999729 |
Target: 5'- ---cACUCGUuGUaGCGCGCGu-CGCGu -3' miRNA: 3'- uguuUGAGUAuUA-CGCGCGUuuGCGC- -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 81238 | 0.66 | 0.999729 |
Target: 5'- -aAGugUCGUGuuUGaCGUGCAAAUGCa -3' miRNA: 3'- ugUUugAGUAUu-AC-GCGCGUUUGCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 104086 | 0.66 | 0.999654 |
Target: 5'- gGCcGGCUCGUugugacgccAUGUGCGgGGGCGCu -3' miRNA: 3'- -UGuUUGAGUAu--------UACGCGCgUUUGCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 48416 | 0.66 | 0.999654 |
Target: 5'- cACGGGgUCA--AUGCGCuGCcAACGCa -3' miRNA: 3'- -UGUUUgAGUauUACGCG-CGuUUGCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 2334 | 0.66 | 0.999561 |
Target: 5'- cGCAAACUaaacuugAUGAaGCGCGCGcuCGCc -3' miRNA: 3'- -UGUUUGAg------UAUUaCGCGCGUuuGCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 128937 | 0.66 | 0.999561 |
Target: 5'- aACGu-CUUAUAuuuuaggGCGCGCGAuCGCGu -3' miRNA: 3'- -UGUuuGAGUAUua-----CGCGCGUUuGCGC- -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 12908 | 0.66 | 0.999561 |
Target: 5'- cACGAGCgCAUAAU-CG-GCAAACGCc -3' miRNA: 3'- -UGUUUGaGUAUUAcGCgCGUUUGCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 52050 | 0.66 | 0.999561 |
Target: 5'- -----gUCGUAGUGaauGCGCAuGACGCGa -3' miRNA: 3'- uguuugAGUAUUACg--CGCGU-UUGCGC- -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 46884 | 0.66 | 0.999561 |
Target: 5'- uCAGGCUgGUGGUG-GCGC--GCGUGg -3' miRNA: 3'- uGUUUGAgUAUUACgCGCGuuUGCGC- -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 45093 | 0.66 | 0.999448 |
Target: 5'- cGCGAcuugcucugccACUCGcgg-GCGCGC-GACGCGu -3' miRNA: 3'- -UGUU-----------UGAGUauuaCGCGCGuUUGCGC- -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 88935 | 0.67 | 0.99931 |
Target: 5'- -aAAGCUUGUGgacAUGCGCGagcACGCGu -3' miRNA: 3'- ugUUUGAGUAU---UACGCGCguuUGCGC- -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 119726 | 0.67 | 0.99931 |
Target: 5'- cACGuGCUCAgcGUGCGuCGCucuaccgaggcuAAACGCa -3' miRNA: 3'- -UGUuUGAGUauUACGC-GCG------------UUUGCGc -5' |
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22942 | 5' | -46.9 | NC_005137.2 | + | 90143 | 0.67 | 0.998943 |
Target: 5'- aACAAAUcgcuauacuuuUCAUAGUuugGCGCGUGcAACGCGu -3' miRNA: 3'- -UGUUUG-----------AGUAUUA---CGCGCGU-UUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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