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miRNA: 3'- ugaUGACGGGa-GGAGaUGCGuCGGCG- -5'
miRNA: 3'- ugaUGACGGGa-------------GGAGAUGc-----GUCGGCG- -5'
miRNA: 3'- -UGAUGACGGGAGGAGAUGCGUCGGCG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 23204 | 3' | -57.5 | NC_005259.1 | + | 28150 | 0.72 | 0.305859 |
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Target: 5'- --aGCUGCCCggCCUCgACGC-GCUGCc -3' miRNA: 3'- ugaUGACGGGa-GGAGaUGCGuCGGCG- -5' |
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| 23204 | 3' | -57.5 | NC_005259.1 | + | 30378 | 0.73 | 0.280063 |
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Target: 5'- --cGCUGCCCggcgagaaaccgggCCUCUACcguagcCAGCCGCa -3' miRNA: 3'- ugaUGACGGGa-------------GGAGAUGc-----GUCGGCG- -5' |
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| 23204 | 3' | -57.5 | NC_005259.1 | + | 59564 | 1.11 | 0.000552 |
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Target: 5'- aACUACUGCCCUCCUCUACGCAGCCGCu -3' miRNA: 3'- -UGAUGACGGGAGGAGAUGCGUCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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