miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23527 3' -48.2 NC_005260.1 + 188195 0.67 0.999828
Target:  5'- cGAGUGUaUGGCUAAuuauGAGaaagcUGAGGu -3'
miRNA:   3'- cCUCGCAaACCGAUUu---CUCa----ACUCCc -5'
23527 3' -48.2 NC_005260.1 + 111434 0.68 0.999651
Target:  5'- cGGAG-GUgUGGCUcAAAG-GUUGAGcGGc -3'
miRNA:   3'- -CCUCgCAaACCGA-UUUCuCAACUC-CC- -5'
23527 3' -48.2 NC_005260.1 + 187470 0.7 0.997041
Target:  5'- -aAGCGUUUGGCUcu-GAGUUGAu-- -3'
miRNA:   3'- ccUCGCAAACCGAuuuCUCAACUccc -5'
23527 3' -48.2 NC_005260.1 + 111013 0.71 0.994469
Target:  5'- uGGA-CucUUGuGCUAGAGAGUUGAGGa -3'
miRNA:   3'- -CCUcGcaAAC-CGAUUUCUCAACUCCc -5'
23527 3' -48.2 NC_005260.1 + 187766 1.15 0.011482
Target:  5'- aGGAGCGUUUGGCUAAAGAGUUGAGGGa -3'
miRNA:   3'- -CCUCGCAAACCGAUUUCUCAACUCCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.