Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23532 | 3' | -32.9 | NC_005260.1 | + | 166154 | 0.66 | 1 |
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Target: 5'- -----aCAGUUGAUCCAAuguccgGAACg- -3' miRNA: 3'- aaaaaaGUUAAUUAGGUUua----UUUGaa -5' |
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| 23532 | 3' | -32.9 | NC_005260.1 | + | 32881 | 0.66 | 1 |
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Target: 5'- ----aUUAAUUGAgUCCAAucAUGAACUUg -3' miRNA: 3'- aaaaaAGUUAAUU-AGGUU--UAUUUGAA- -5' |
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| 23532 | 3' | -32.9 | NC_005260.1 | + | 60983 | 0.66 | 1 |
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Target: 5'- ----aUCAGUUGacaacaguuugaGUCCAGAUAuACUg -3' miRNA: 3'- aaaaaAGUUAAU------------UAGGUUUAUuUGAa -5' |
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| 23532 | 3' | -32.9 | NC_005260.1 | + | 223392 | 0.67 | 1 |
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Target: 5'- ---gUUCGGUauAUCCAGaAUAAGCUa -3' miRNA: 3'- aaaaAAGUUAauUAGGUU-UAUUUGAa -5' |
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| 23532 | 3' | -32.9 | NC_005260.1 | + | 129299 | 0.71 | 1 |
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Target: 5'- -aUUUUCAAUUGAgCCu-GUGAACUUc -3' miRNA: 3'- aaAAAAGUUAAUUaGGuuUAUUUGAA- -5' |
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| 23532 | 3' | -32.9 | NC_005260.1 | + | 194358 | 0.73 | 1 |
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Target: 5'- ----aUCAGgccgUGAUCCAAcgAGACUUg -3' miRNA: 3'- aaaaaAGUUa---AUUAGGUUuaUUUGAA- -5' |
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| 23532 | 3' | -32.9 | NC_005260.1 | + | 133327 | 0.75 | 1 |
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Target: 5'- gUUUUUC--UUGAUCCAGAUAGAUa- -3' miRNA: 3'- aAAAAAGuuAAUUAGGUUUAUUUGaa -5' |
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| 23532 | 3' | -32.9 | NC_005260.1 | + | 155266 | 0.76 | 1 |
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Target: 5'- ----aUCAA--GAUCCAAAUAAACUg -3' miRNA: 3'- aaaaaAGUUaaUUAGGUUUAUUUGAa -5' |
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| 23532 | 3' | -32.9 | NC_005260.1 | + | 104087 | 0.68 | 1 |
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Target: 5'- cUUUUUCAGUUGAUCgCGAAcuuuAGCa- -3' miRNA: 3'- aAAAAAGUUAAUUAG-GUUUau--UUGaa -5' |
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| 23532 | 3' | -32.9 | NC_005260.1 | + | 57805 | 1.04 | 0.847299 |
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Target: 5'- cUUUUUUCAAUUAAUCCAAAUAAACUUu -3' miRNA: 3'- -AAAAAAGUUAAUUAGGUUUAUUUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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