miRNA: 3'- -UGGaaCCAaUUACAUCGCUGG--------------CCGcg -5'
miRNA: 3'- -UGGAACCAAUuacAUCGCuggCCGCG- -5'
miRNA: 3'- -UGGAACCAa-UUAcaUCGCUGGCCgCG- -5'
miRNA: 3'- -UGGaa--CCAAUUACA---UCGCUGGC-------CGCG- -5'
miRNA: 3'- -UGGAACCAAUUACAUCGCUGGCCGCG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2453 | 3' | -51.7 | NC_001434.1 | + | 2218 | 0.69 | 0.193856 |
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Target: 5'- cGCCggGGUaAGUGUGGCGGCCcuacuagguaagaaGGCu- -3' miRNA: 3'- -UGGaaCCAaUUACAUCGCUGG--------------CCGcg -5' |
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| 2453 | 3' | -51.7 | NC_001434.1 | + | 5895 | 0.69 | 0.189536 |
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Target: 5'- aACCUUGGUUAcgaUAGUGua-GGCGCu -3' miRNA: 3'- -UGGAACCAAUuacAUCGCuggCCGCG- -5' |
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| 2453 | 3' | -51.7 | NC_001434.1 | + | 6679 | 0.76 | 0.058118 |
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Target: 5'- gACCUUGGUccAGUcgAGCGACCGG-GCa -3' miRNA: 3'- -UGGAACCAa-UUAcaUCGCUGGCCgCG- -5' |
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| 2453 | 3' | -51.7 | NC_001434.1 | + | 2553 | 0.78 | 0.039411 |
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Target: 5'- gGCCggggGGUUAAUGUauaGGCGGCCGcgccgucGCGCa -3' miRNA: 3'- -UGGaa--CCAAUUACA---UCGCUGGC-------CGCG- -5' |
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| 2453 | 3' | -51.7 | NC_001434.1 | + | 6610 | 1.13 | 5.9e-05 |
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Target: 5'- gACCUUGGUUAAUGUAGCGACCGGCGCg -3' miRNA: 3'- -UGGAACCAAUUACAUCGCUGGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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