Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25131 | 3' | -55.5 | NC_005336.1 | + | 49501 | 0.66 | 0.917008 |
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Target: 5'- cGUCGuuGGUGcUCacuagcaccaGCGUGCCGGGc- -3' miRNA: 3'- -CAGCuuCCACaAGg---------UGUACGGCCCcu -5' |
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| 25131 | 3' | -55.5 | NC_005336.1 | + | 48675 | 0.66 | 0.911086 |
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Target: 5'- aUgGAAGaGUGcgCCucucUGCCGGGGAg -3' miRNA: 3'- cAgCUUC-CACaaGGugu-ACGGCCCCU- -5' |
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| 25131 | 3' | -55.5 | NC_005336.1 | + | 84591 | 0.66 | 0.898523 |
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Target: 5'- -gCGuGAGcGUGUUCCGgAUGUaguCGGGGAu -3' miRNA: 3'- caGC-UUC-CACAAGGUgUACG---GCCCCU- -5' |
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| 25131 | 3' | -55.5 | NC_005336.1 | + | 72681 | 0.67 | 0.855312 |
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Target: 5'- cUCGAAGGUGggCgC-CAUGCgCGGGa- -3' miRNA: 3'- cAGCUUCCACaaG-GuGUACG-GCCCcu -5' |
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| 25131 | 3' | -55.5 | NC_005336.1 | + | 104268 | 0.68 | 0.839201 |
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Target: 5'- uUCG-AGGUcccagaGUUCCGCGggaagGCCGGcGGAc -3' miRNA: 3'- cAGCuUCCA------CAAGGUGUa----CGGCC-CCU- -5' |
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| 25131 | 3' | -55.5 | NC_005336.1 | + | 18703 | 0.68 | 0.822324 |
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Target: 5'- uGUCGAGGaag-UCCACGacGUCGGGGAa -3' miRNA: 3'- -CAGCUUCcacaAGGUGUa-CGGCCCCU- -5' |
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| 25131 | 3' | -55.5 | NC_005336.1 | + | 93376 | 0.68 | 0.813617 |
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Target: 5'- gGUUGGAGGcgcgGcgCCGCGcGCCGGuGGAa -3' miRNA: 3'- -CAGCUUCCa---CaaGGUGUaCGGCC-CCU- -5' |
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| 25131 | 3' | -55.5 | NC_005336.1 | + | 40477 | 0.69 | 0.774374 |
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Target: 5'- cUCGAAGGUGUggaagagccgcgcgUCCGCGUGCauGGa- -3' miRNA: 3'- cAGCUUCCACA--------------AGGUGUACGgcCCcu -5' |
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| 25131 | 3' | -55.5 | NC_005336.1 | + | 23548 | 0.69 | 0.762005 |
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Target: 5'- -aCGAcccGGUGcucuguuuccugguuUUCUACGUGCCGGGGc -3' miRNA: 3'- caGCUu--CCAC---------------AAGGUGUACGGCCCCu -5' |
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| 25131 | 3' | -55.5 | NC_005336.1 | + | 82064 | 1.08 | 0.003291 |
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Target: 5'- uGUCGAAGGUGUUCCACAUGCCGGGGAc -3' miRNA: 3'- -CAGCUUCCACAAGGUGUACGGCCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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