Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2625 | 3' | -48.3 | NC_001491.2 | + | 30051 | 0.66 | 0.999272 |
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Target: 5'- gCGCCguguuacgucgauggGGUGGGGCcg-GCGCGUGUGu- -3' miRNA: 3'- -GUGG---------------CCACCCUGcaaUGUGUAUAUuu -5' |
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| 2625 | 3' | -48.3 | NC_001491.2 | + | 112859 | 0.67 | 0.998239 |
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Target: 5'- -cUCGGUGGGACc--GCACGUAa--- -3' miRNA: 3'- guGGCCACCCUGcaaUGUGUAUauuu -5' |
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| 2625 | 3' | -48.3 | NC_001491.2 | + | 61430 | 0.68 | 0.994221 |
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Target: 5'- -cCUGGgguaGGGACGUUACACGa----- -3' miRNA: 3'- guGGCCa---CCCUGCAAUGUGUauauuu -5' |
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| 2625 | 3' | -48.3 | NC_001491.2 | + | 95638 | 0.68 | 0.993283 |
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Target: 5'- --aUGGUGGGGCGgaaaGCACGUGUc-- -3' miRNA: 3'- gugGCCACCCUGCaa--UGUGUAUAuuu -5' |
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| 2625 | 3' | -48.3 | NC_001491.2 | + | 30189 | 0.69 | 0.988249 |
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Target: 5'- gCGCCcucGUGcGGACGUguaugaagGCGCAUGUAAAa -3' miRNA: 3'- -GUGGc--CAC-CCUGCAa-------UGUGUAUAUUU- -5' |
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| 2625 | 3' | -48.3 | NC_001491.2 | + | 147353 | 1.07 | 0.018307 |
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Target: 5'- aCACCGGUGGGACGUUACACAUAUAAAa -3' miRNA: 3'- -GUGGCCACCCUGCAAUGUGUAUAUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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