miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27285 3' -50 NC_005856.1 + 31106 0.66 0.967537
Target:  5'- uGCAGUgaCAGcUGGCGCggUGGCGUa-- -3'
miRNA:   3'- cCGUUA--GUC-ACCGUGaaACCGUAcuu -5'
27285 3' -50 NC_005856.1 + 37013 0.67 0.953579
Target:  5'- uGGCGuuccuGUCAGacucgggcuuucuauUGGCGCUUUGGUAUc-- -3'
miRNA:   3'- -CCGU-----UAGUC---------------ACCGUGAAACCGUAcuu -5'
27285 3' -50 NC_005856.1 + 17474 0.7 0.87759
Target:  5'- gGGCGAU-AGUGGCAUggcUGGCgAUGGg -3'
miRNA:   3'- -CCGUUAgUCACCGUGaa-ACCG-UACUu -5'
27285 3' -50 NC_005856.1 + 84559 0.7 0.861329
Target:  5'- aGGUuuUCAGUGGUACgg-GGC-UGAu -3'
miRNA:   3'- -CCGuuAGUCACCGUGaaaCCGuACUu -5'
27285 3' -50 NC_005856.1 + 29550 0.73 0.712996
Target:  5'- cGCGAUCGGcGGCGCUgaaaUUGGCcgGGc -3'
miRNA:   3'- cCGUUAGUCaCCGUGA----AACCGuaCUu -5'
27285 3' -50 NC_005856.1 + 8358 1.1 0.004598
Target:  5'- aGGCAAUCAGUGGCACUUUGGCAUGAAg -3'
miRNA:   3'- -CCGUUAGUCACCGUGAAACCGUACUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.