miRNA display CGI


Results 1 - 16 of 16 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27326 3' -46.3 NC_005856.1 + 28159 0.66 0.999522
Target:  5'- aCUUCGGCCuGCAAUgcaUUGAuCAUGCu-- -3'
miRNA:   3'- -GGAGUUGG-CGUUA---AACUuGUACGcug -5'
27326 3' -46.3 NC_005856.1 + 73632 0.66 0.999397
Target:  5'- aUCUUuuCCGCAAUUgccGAccAgGUGUGACa -3'
miRNA:   3'- -GGAGuuGGCGUUAAa--CU--UgUACGCUG- -5'
27326 3' -46.3 NC_005856.1 + 15285 0.67 0.998842
Target:  5'- aCUgGACCGUcgg-UGAGCGUGgCGAg -3'
miRNA:   3'- gGAgUUGGCGuuaaACUUGUAC-GCUg -5'
27326 3' -46.3 NC_005856.1 + 29052 0.67 0.998842
Target:  5'- gCUCAAUCGCAcuggUGGGC--GCGAUa -3'
miRNA:   3'- gGAGUUGGCGUuaa-ACUUGuaCGCUG- -5'
27326 3' -46.3 NC_005856.1 + 36684 0.67 0.998266
Target:  5'- aCUUUAACCGCGcUUgcUGGucuUGCGACa -3'
miRNA:   3'- -GGAGUUGGCGUuAA--ACUuguACGCUG- -5'
27326 3' -46.3 NC_005856.1 + 21968 0.68 0.996382
Target:  5'- aCCUCAugcagauugcGCCGCuGUUUGA--GUGUGAa -3'
miRNA:   3'- -GGAGU----------UGGCGuUAAACUugUACGCUg -5'
27326 3' -46.3 NC_005856.1 + 57729 0.68 0.99494
Target:  5'- gCUCAGCCGCGAUaacGAACugacuUGgGAa -3'
miRNA:   3'- gGAGUUGGCGUUAaa-CUUGu----ACgCUg -5'
27326 3' -46.3 NC_005856.1 + 67006 0.69 0.993065
Target:  5'- aCCUCGAucCCGCAAcgguacuuUUUGAguucgaagGCGUGCGcCg -3'
miRNA:   3'- -GGAGUU--GGCGUU--------AAACU--------UGUACGCuG- -5'
27326 3' -46.3 NC_005856.1 + 90779 0.7 0.985915
Target:  5'- uUUCAGCCGCAAUgUGAucaccuGCAUGuUGAUc -3'
miRNA:   3'- gGAGUUGGCGUUAaACU------UGUAC-GCUG- -5'
27326 3' -46.3 NC_005856.1 + 92426 0.71 0.974144
Target:  5'- uUCUUggUCGUGAUgaggUUGAGCggGCGACg -3'
miRNA:   3'- -GGAGuuGGCGUUA----AACUUGuaCGCUG- -5'
27326 3' -46.3 NC_005856.1 + 56309 0.71 0.971431
Target:  5'- aUCUCAACCGCugcguaccuGCGUGCGGu -3'
miRNA:   3'- -GGAGUUGGCGuuaaacu--UGUACGCUg -5'
27326 3' -46.3 NC_005856.1 + 82774 0.71 0.971117
Target:  5'- aCUCAaugaaaugACCGCGAUggUUGAAC-UGCGGa -3'
miRNA:   3'- gGAGU--------UGGCGUUA--AACUUGuACGCUg -5'
27326 3' -46.3 NC_005856.1 + 60214 0.72 0.960528
Target:  5'- gCCUUGACgGU--UUUGAACGcUGCGGCg -3'
miRNA:   3'- -GGAGUUGgCGuuAAACUUGU-ACGCUG- -5'
27326 3' -46.3 NC_005856.1 + 82654 0.72 0.952135
Target:  5'- gCUUCAGCUGCAAaUUGAACAUuuGAa -3'
miRNA:   3'- -GGAGUUGGCGUUaAACUUGUAcgCUg -5'
27326 3' -46.3 NC_005856.1 + 37414 0.83 0.500762
Target:  5'- aUUCAGCCGCA---UGAAUAUGUGACg -3'
miRNA:   3'- gGAGUUGGCGUuaaACUUGUACGCUG- -5'
27326 3' -46.3 NC_005856.1 + 40734 1.14 0.007535
Target:  5'- gCCUCAACCGCAAUUUGAACAUGCGACa -3'
miRNA:   3'- -GGAGUUGGCGUUAAACUUGUACGCUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.