Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27358 | 3' | -48.7 | NC_005857.1 | + | 15904 | 0.66 | 0.939893 |
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Target: 5'- --cGCCAACAgcGUCAaaaUCGGCGAUGu- -3' miRNA: 3'- auaUGGUUGUa-CAGU---GGCCGUUACuu -5' |
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| 27358 | 3' | -48.7 | NC_005857.1 | + | 32120 | 0.66 | 0.939893 |
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Target: 5'- uUGUACCAAC---UCACgGGCAAccUGGAg -3' miRNA: 3'- -AUAUGGUUGuacAGUGgCCGUU--ACUU- -5' |
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| 27358 | 3' | -48.7 | NC_005857.1 | + | 35177 | 0.68 | 0.877785 |
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Target: 5'- -uUACCGcgugGCcgGUCGCUGGCAGcGAu -3' miRNA: 3'- auAUGGU----UGuaCAGUGGCCGUUaCUu -5' |
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| 27358 | 3' | -48.7 | NC_005857.1 | + | 5534 | 0.7 | 0.802706 |
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Target: 5'- cAUACCAAUugggGUGUCGCCGGUg----- -3' miRNA: 3'- aUAUGGUUG----UACAGUGGCCGuuacuu -5' |
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| 27358 | 3' | -48.7 | NC_005857.1 | + | 8461 | 0.72 | 0.667554 |
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Target: 5'- --cGCCAGCAUuagCACCGGCAGUa-- -3' miRNA: 3'- auaUGGUUGUAca-GUGGCCGUUAcuu -5' |
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| 27358 | 3' | -48.7 | NC_005857.1 | + | 1967 | 1.05 | 0.006035 |
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Target: 5'- cUAUACCAACAUGUCACCGGCAAUGAAg -3' miRNA: 3'- -AUAUGGUUGUACAGUGGCCGUUACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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