Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27439 | 3' | -53.7 | NC_005869.1 | + | 15961 | 0.66 | 0.621022 |
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Target: 5'- cAGCGA-CGGGUGCUGGuGcuggGCAcgcgCCc -3' miRNA: 3'- -UCGCUaGUCCACGACCuCa---UGUa---GGu -5' |
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| 27439 | 3' | -53.7 | NC_005869.1 | + | 33049 | 0.67 | 0.586205 |
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Target: 5'- uGGCGGgaaUAGGcaGCaGGGGUACGUCCc -3' miRNA: 3'- -UCGCUa--GUCCa-CGaCCUCAUGUAGGu -5' |
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| 27439 | 3' | -53.7 | NC_005869.1 | + | 8955 | 0.67 | 0.56319 |
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Target: 5'- aGGUGAggaAGGUGUgcaugcagUGGAGUGCA-CCGu -3' miRNA: 3'- -UCGCUag-UCCACG--------ACCUCAUGUaGGU- -5' |
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| 27439 | 3' | -53.7 | NC_005869.1 | + | 1854 | 0.68 | 0.485034 |
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Target: 5'- uGGCGGagCAGGUGCggcagGGGcuGUGCAUCa- -3' miRNA: 3'- -UCGCUa-GUCCACGa----CCU--CAUGUAGgu -5' |
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| 27439 | 3' | -53.7 | NC_005869.1 | + | 17484 | 0.74 | 0.22646 |
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Target: 5'- gGGCGGcgUGGGUGCUGGAGcggGCGgcUCCAc -3' miRNA: 3'- -UCGCUa-GUCCACGACCUCa--UGU--AGGU- -5' |
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| 27439 | 3' | -53.7 | NC_005869.1 | + | 1557 | 1.09 | 0.000648 |
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Target: 5'- gAGCGAUCAGGUGCUGGAGUACAUCCAg -3' miRNA: 3'- -UCGCUAGUCCACGACCUCAUGUAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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