Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27443 | 5' | -56.8 | NC_005869.1 | + | 5040 | 0.66 | 0.491545 |
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Target: 5'- -aGGUCCugcaggaGGUGUcGGCCUcCCAGGGa -3' miRNA: 3'- caCCGGGug-----CUACAuCUGGA-GGUCCU- -5' |
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| 27443 | 5' | -56.8 | NC_005869.1 | + | 18403 | 0.66 | 0.464359 |
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Target: 5'- -cGGUCCACGgcGUGGgagacggccugguugGCCacgUCCAGGGc -3' miRNA: 3'- caCCGGGUGCuaCAUC---------------UGG---AGGUCCU- -5' |
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| 27443 | 5' | -56.8 | NC_005869.1 | + | 28703 | 0.66 | 0.450048 |
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Target: 5'- uUGGCUCAUcguaGAUGUGGuCCUCggcgCAGGGu -3' miRNA: 3'- cACCGGGUG----CUACAUCuGGAG----GUCCU- -5' |
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| 27443 | 5' | -56.8 | NC_005869.1 | + | 7956 | 0.67 | 0.439976 |
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Target: 5'- -cGGCCgugccaGGUGUAGGUCUCCAGGu -3' miRNA: 3'- caCCGGgug---CUACAUCUGGAGGUCCu -5' |
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| 27443 | 5' | -56.8 | NC_005869.1 | + | 8361 | 0.67 | 0.410553 |
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Target: 5'- cUGGCCCGCGccGccGGGCCcaccuucgUCCAGGu -3' miRNA: 3'- cACCGGGUGCuaCa-UCUGG--------AGGUCCu -5' |
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| 27443 | 5' | -56.8 | NC_005869.1 | + | 20749 | 0.69 | 0.335858 |
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Target: 5'- -cGGCaCCugGAUGUGGaacuugcaguagcgGCCguugcCCAGGAg -3' miRNA: 3'- caCCG-GGugCUACAUC--------------UGGa----GGUCCU- -5' |
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| 27443 | 5' | -56.8 | NC_005869.1 | + | 20061 | 0.69 | 0.330032 |
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Target: 5'- -aGGCCUcucagcucaacGCGGUGgucGACCUgCAGGAc -3' miRNA: 3'- caCCGGG-----------UGCUACau-CUGGAgGUCCU- -5' |
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| 27443 | 5' | -56.8 | NC_005869.1 | + | 16995 | 0.69 | 0.321837 |
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Target: 5'- gGUGGCCCggagcACGGgcaUGGccaccaggggcGCCUCCAGGAc -3' miRNA: 3'- -CACCGGG-----UGCUac-AUC-----------UGGAGGUCCU- -5' |
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| 27443 | 5' | -56.8 | NC_005869.1 | + | 7573 | 1.08 | 0.000378 |
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Target: 5'- uGUGGCCCACGAUGUAGACCUCCAGGAa -3' miRNA: 3'- -CACCGGGUGCUACAUCUGGAGGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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