miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27484 5' -66 NC_005872.1 + 1328 0.87 0.002357
Target:  5'- uGGGAGGCCGCGGCCUCCCucccgacAAGGGa- -3'
miRNA:   3'- uCCCUCCGGCGCCGGAGGG-------UUCCCgc -5'
27484 5' -66 NC_005872.1 + 26674 0.72 0.046323
Target:  5'- aAGGGcGGGCaCGUGGCUuuuUCCUAAgGGGCGc -3'
miRNA:   3'- -UCCC-UCCG-GCGCCGG---AGGGUU-CCCGC- -5'
27484 5' -66 NC_005872.1 + 20591 0.66 0.146383
Target:  5'- gAGGGcuaAGGCUGa-GCCUCCUAGGgaGGUGg -3'
miRNA:   3'- -UCCC---UCCGGCgcCGGAGGGUUC--CCGC- -5'
27484 5' -66 NC_005872.1 + 1376 1.07 0.00005
Target:  5'- gAGGGAGGCCGCGGCCUCCCAAGGGCGu -3'
miRNA:   3'- -UCCCUCCGGCGCCGGAGGGUUCCCGC- -5'
27484 5' -66 NC_005872.1 + 3266 0.75 0.023837
Target:  5'- cGGGGGGGaaacgCGCGGCC-CCUAAGGGgGc -3'
miRNA:   3'- -UCCCUCCg----GCGCCGGaGGGUUCCCgC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.