miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27490 5' -56.3 NC_005872.1 + 14095 1.1 0.00026
Target:  5'- gGGAGUGGGUGGUCAAGCACCCACGACc -3'
miRNA:   3'- -CCUCACCCACCAGUUCGUGGGUGCUG- -5'
27490 5' -56.3 NC_005872.1 + 11333 0.69 0.309171
Target:  5'- uGGGGcugGGGUGGUUGccguuAGUcaaGCUCACGACu -3'
miRNA:   3'- -CCUCa--CCCACCAGU-----UCG---UGGGUGCUG- -5'
27490 5' -56.3 NC_005872.1 + 5996 0.69 0.31721
Target:  5'- uGAGUGaGGUGGcUGAGgGCCgCAUGACu -3'
miRNA:   3'- cCUCAC-CCACCaGUUCgUGG-GUGCUG- -5'
27490 5' -56.3 NC_005872.1 + 24799 0.67 0.424679
Target:  5'- cGGGUGGGUGG-CAuuGGUuagGCCCauugaaaACGGCu -3'
miRNA:   3'- cCUCACCCACCaGU--UCG---UGGG-------UGCUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.