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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27494 | 3' | -51.6 | NC_005872.1 | + | 18151 | 0.71 | 0.370861 |
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Target: 5'- cGUGGCUGUGCCGauauacuacaAGGUgucuGAGuCCUCUGu -3' miRNA: 3'- -CACCGAUACGGCa---------UCUA----UUC-GGAGAU- -5' |
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| 27494 | 3' | -51.6 | NC_005872.1 | + | 25686 | 0.7 | 0.442287 |
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Target: 5'- aUGGCUAUGUCGUGGcGUAAGgCUg-- -3' miRNA: 3'- cACCGAUACGGCAUC-UAUUCgGAgau -5' |
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| 27494 | 3' | -51.6 | NC_005872.1 | + | 8178 | 0.66 | 0.645571 |
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Target: 5'- gGUGGCUAgggugcucucuaagaUGCggCGUAGAUAGGCUUa-- -3' miRNA: 3'- -CACCGAU---------------ACG--GCAUCUAUUCGGAgau -5' |
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| 27494 | 3' | -51.6 | NC_005872.1 | + | 16085 | 0.66 | 0.664966 |
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Target: 5'- -cGGCUAgGCUGUuuAGAUGacAGCCUUa- -3' miRNA: 3'- caCCGAUaCGGCA--UCUAU--UCGGAGau -5' |
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| 27494 | 3' | -51.6 | NC_005872.1 | + | 13552 | 0.66 | 0.677043 |
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Target: 5'- -aGGCUuauuucuauUGCUG-AGAagGAGCCUCUAg -3' miRNA: 3'- caCCGAu--------ACGGCaUCUa-UUCGGAGAU- -5' |
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| 27494 | 3' | -51.6 | NC_005872.1 | + | 20706 | 1.06 | 0.0011 |
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Target: 5'- uGUGGCUAUGCCGUAGAUAAGCCUCUAc -3' miRNA: 3'- -CACCGAUACGGCAUCUAUUCGGAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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