Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27515 | 3' | -46.4 | NC_005879.1 | + | 8918 | 0.66 | 0.987597 |
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Target: 5'- uCCUGCUGAGgGAcUGAAGuUAUGGUCg -3' miRNA: 3'- -GGGUGGUUCaCUaACUUUuGUACCGG- -5' |
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| 27515 | 3' | -46.4 | NC_005879.1 | + | 33781 | 0.66 | 0.987597 |
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Target: 5'- gCCCAUCGGGUG-UUGGuagauuucCGUGGUg -3' miRNA: 3'- -GGGUGGUUCACuAACUuuu-----GUACCGg -5' |
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| 27515 | 3' | -46.4 | NC_005879.1 | + | 10221 | 0.68 | 0.962268 |
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Target: 5'- gCCCGCaGAGU--UUGAGAACA-GGCa -3' miRNA: 3'- -GGGUGgUUCAcuAACUUUUGUaCCGg -5' |
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| 27515 | 3' | -46.4 | NC_005879.1 | + | 30037 | 0.69 | 0.932595 |
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Target: 5'- -aCGCCAAGUacAUUGAAGgcgcggaagaagGCAUGGCUu -3' miRNA: 3'- ggGUGGUUCAc-UAACUUU------------UGUACCGG- -5' |
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| 27515 | 3' | -46.4 | NC_005879.1 | + | 28316 | 0.7 | 0.913418 |
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Target: 5'- -aCGCCGAGuUGAcuUUGGAAuuGUGGCUg -3' miRNA: 3'- ggGUGGUUC-ACU--AACUUUugUACCGG- -5' |
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| 27515 | 3' | -46.4 | NC_005879.1 | + | 8580 | 0.7 | 0.89134 |
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Target: 5'- uUCAaaAAGUacgGGUUGAAGGCAUGGCg -3' miRNA: 3'- gGGUggUUCA---CUAACUUUUGUACCGg -5' |
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| 27515 | 3' | -46.4 | NC_005879.1 | + | 30830 | 0.71 | 0.875064 |
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Target: 5'- aCCCACCAcAG-GAgcGAAAACAUGaGUg -3' miRNA: 3'- -GGGUGGU-UCaCUaaCUUUUGUAC-CGg -5' |
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| 27515 | 3' | -46.4 | NC_005879.1 | + | 18921 | 0.76 | 0.579137 |
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Target: 5'- aCC-CUAAGcuuugGAUUGAAGGCAUGGCa -3' miRNA: 3'- gGGuGGUUCa----CUAACUUUUGUACCGg -5' |
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| 27515 | 3' | -46.4 | NC_005879.1 | + | 23584 | 1.17 | 0.001867 |
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Target: 5'- gCCCACCAAGUGAUUGAAAACAUGGCCa -3' miRNA: 3'- -GGGUGGUUCACUAACUUUUGUACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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