Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28558 | 5' | -56.6 | NC_005946.1 | + | 42359 | 0.66 | 0.832807 |
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Target: 5'- aCCCuuuGgGCCGUCG-GGCCccGCGUCg- -3' miRNA: 3'- -GGGc--UgUGGCAGCuCCGGu-UGCAGau -5' |
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| 28558 | 5' | -56.6 | NC_005946.1 | + | 19601 | 0.66 | 0.82431 |
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Target: 5'- cCCCGAC-CUGUCu--GCCGcaGCGUCUGu -3' miRNA: 3'- -GGGCUGuGGCAGcucCGGU--UGCAGAU- -5' |
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| 28558 | 5' | -56.6 | NC_005946.1 | + | 81255 | 0.67 | 0.797781 |
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Target: 5'- cCCCaGCACCcUCGAcGCCcuaaAGCGUCUGg -3' miRNA: 3'- -GGGcUGUGGcAGCUcCGG----UUGCAGAU- -5' |
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| 28558 | 5' | -56.6 | NC_005946.1 | + | 103837 | 0.67 | 0.769878 |
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Target: 5'- --aGGCgACCGUCGAGGgCGGCagaGUCUGg -3' miRNA: 3'- gggCUG-UGGCAGCUCCgGUUG---CAGAU- -5' |
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| 28558 | 5' | -56.6 | NC_005946.1 | + | 4957 | 0.68 | 0.750635 |
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Target: 5'- cUCCGACAacuaCGgCGAGGCCGAuCGUa-- -3' miRNA: 3'- -GGGCUGUg---GCaGCUCCGGUU-GCAgau -5' |
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| 28558 | 5' | -56.6 | NC_005946.1 | + | 15925 | 0.69 | 0.690655 |
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Target: 5'- gCCGAgGCgGUCGuGGCCAugGa--- -3' miRNA: 3'- gGGCUgUGgCAGCuCCGGUugCagau -5' |
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| 28558 | 5' | -56.6 | NC_005946.1 | + | 95317 | 0.69 | 0.690655 |
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Target: 5'- cCCCGGCGcCCGUggaCGAGGCCAccagagagGCGg--- -3' miRNA: 3'- -GGGCUGU-GGCA---GCUCCGGU--------UGCagau -5' |
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| 28558 | 5' | -56.6 | NC_005946.1 | + | 72732 | 0.72 | 0.487653 |
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Target: 5'- gUCCGGCGCUG-CGGGGUCcGCGUCc- -3' miRNA: 3'- -GGGCUGUGGCaGCUCCGGuUGCAGau -5' |
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| 28558 | 5' | -56.6 | NC_005946.1 | + | 78584 | 0.74 | 0.405052 |
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Target: 5'- cCCCGAgGCCGUCuuuGAGGCCAuuccggcucACGUUc- -3' miRNA: 3'- -GGGCUgUGGCAG---CUCCGGU---------UGCAGau -5' |
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| 28558 | 5' | -56.6 | NC_005946.1 | + | 60028 | 0.77 | 0.268819 |
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Target: 5'- gCCUGuguCGCCGUCGAGGUaAGCGUCUc -3' miRNA: 3'- -GGGCu--GUGGCAGCUCCGgUUGCAGAu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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