Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30348 | 5' | -52.4 | NC_006431.1 | + | 3340 | 0.66 | 0.336574 |
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Target: 5'- gGCugCCCU----GGAGGGCCUacccccccguuuccUCUUCa -3' miRNA: 3'- -CGugGGGGaguuCCUUUCGGA--------------AGAAG- -5' |
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| 30348 | 5' | -52.4 | NC_006431.1 | + | 5414 | 0.67 | 0.276689 |
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Target: 5'- aCACCUCCUCAGGGuugaGGAGCUgggCgUCc -3' miRNA: 3'- cGUGGGGGAGUUCC----UUUCGGaa-GaAG- -5' |
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| 30348 | 5' | -52.4 | NC_006431.1 | + | 3682 | 0.67 | 0.260542 |
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Target: 5'- aCACUCCCUCAuGGAGGuUCUUCaUCu -3' miRNA: 3'- cGUGGGGGAGUuCCUUUcGGAAGaAG- -5' |
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| 30348 | 5' | -52.4 | NC_006431.1 | + | 8249 | 0.69 | 0.203543 |
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Target: 5'- uGCGCCCCCUCcucuGGucAGCCa----- -3' miRNA: 3'- -CGUGGGGGAGuu--CCuuUCGGaagaag -5' |
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| 30348 | 5' | -52.4 | NC_006431.1 | + | 4046 | 0.69 | 0.197231 |
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Target: 5'- uGCACUUUCa-GGGGGGAGCCUUCUg- -3' miRNA: 3'- -CGUGGGGGagUUCCUUUCGGAAGAag -5' |
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| 30348 | 5' | -52.4 | NC_006431.1 | + | 5538 | 1.12 | 8.4e-05 |
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Target: 5'- cGCACCCCCUCAAGGAAAGCCUUCUUCc -3' miRNA: 3'- -CGUGGGGGAGUUCCUUUCGGAAGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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