Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30582 | 5' | -59.1 | NC_006549.1 | + | 100703 | 0.66 | 0.739195 |
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Target: 5'- uGGGGCugGCGa-CCAA-AGCGCGCu- -3' miRNA: 3'- -UCUCGugCGCcaGGUUgUCGCGCGca -5' |
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| 30582 | 5' | -59.1 | NC_006549.1 | + | 7793 | 0.66 | 0.729501 |
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Target: 5'- aAGGGCGaaaaGgGaccGUCCGGCAGCGCGUc- -3' miRNA: 3'- -UCUCGUg---CgC---CAGGUUGUCGCGCGca -5' |
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| 30582 | 5' | -59.1 | NC_006549.1 | + | 98905 | 0.66 | 0.729501 |
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Target: 5'- uGGA-CGCGCaGGaUCCAucCAGCGUGUGUa -3' miRNA: 3'- -UCUcGUGCG-CC-AGGUu-GUCGCGCGCA- -5' |
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| 30582 | 5' | -59.1 | NC_006549.1 | + | 60457 | 0.67 | 0.699962 |
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Target: 5'- cGGcGUugGCGGUgugcgCCGAUAGCGUGgGg -3' miRNA: 3'- -UCuCGugCGCCA-----GGUUGUCGCGCgCa -5' |
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| 30582 | 5' | -59.1 | NC_006549.1 | + | 32896 | 0.72 | 0.427042 |
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Target: 5'- cGAGCguuGCGGUCgCuGCAGUGUGCGUg -3' miRNA: 3'- uCUCGug-CGCCAG-GuUGUCGCGCGCA- -5' |
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| 30582 | 5' | -59.1 | NC_006549.1 | + | 107459 | 0.72 | 0.384502 |
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Target: 5'- uGGGCGCGCGuguGUCCGACuuuGCGaGCGUa -3' miRNA: 3'- uCUCGUGCGC---CAGGUUGu--CGCgCGCA- -5' |
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| 30582 | 5' | -59.1 | NC_006549.1 | + | 52724 | 0.76 | 0.227144 |
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Target: 5'- cGGAGCGCGCGG-CC-AUGGCGgGCGa -3' miRNA: 3'- -UCUCGUGCGCCaGGuUGUCGCgCGCa -5' |
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| 30582 | 5' | -59.1 | NC_006549.1 | + | 10417 | 1.07 | 0.00167 |
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Target: 5'- aAGAGCACGCGGUCCAACAGCGCGCGUa -3' miRNA: 3'- -UCUCGUGCGCCAGGUUGUCGCGCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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