miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31416 3' -53 NC_006635.1 + 26810 0.68 0.518095
Target:  5'- aCGCccucuugGCUUugcaaaGCCAAAAGGGCGUGu -3'
miRNA:   3'- -GCGca-----CGGAuua---CGGUUUUCCCGCAU- -5'
31416 3' -53 NC_006635.1 + 26872 0.68 0.473529
Target:  5'- aCGCccuuuugGCUUugcaaaGCCAAGAGGGCGUAu -3'
miRNA:   3'- -GCGca-----CGGAuua---CGGUUUUCCCGCAU- -5'
31416 3' -53 NC_006635.1 + 24730 0.73 0.255907
Target:  5'- aGgGUuCC--AUGCCAAAAGGGCGUAu -3'
miRNA:   3'- gCgCAcGGauUACGGUUUUCCCGCAU- -5'
31416 3' -53 NC_006635.1 + 26706 0.74 0.204115
Target:  5'- --aGUGaUCUAAaGCCAAAAGGGCGUAu -3'
miRNA:   3'- gcgCAC-GGAUUaCGGUUUUCCCGCAU- -5'
31416 3' -53 NC_006635.1 + 24688 1.07 0.000884
Target:  5'- uCGCGUGCCUAAUGCCAAAAGGGCGUAu -3'
miRNA:   3'- -GCGCACGGAUUACGGUUUUCCCGCAU- -5'
31416 3' -53 NC_006635.1 + 26909 1.07 0.000884
Target:  5'- uCGCGUGCCUAAUGCCAAAAGGGCGUAu -3'
miRNA:   3'- -GCGCACGGAUUACGGUUUUCCCGCAU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.