Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31422 | 3' | -37.7 | NC_006635.1 | + | 9470 | 0.74 | 0.996571 |
Target: 5'- aGAGAGGGAAAcgucauggguacgUGUACGCCCUCUg-- -3' miRNA: 3'- -UUUUUUUUUUa------------AUAUGCGGGAGAacc -5' |
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31422 | 3' | -37.7 | NC_006635.1 | + | 26746 | 0.75 | 0.991844 |
Target: 5'- ------cAAGUUAUACGCCCUUUUa- -3' miRNA: 3'- uuuuuuuUUUAAUAUGCGGGAGAAcc -5' |
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31422 | 3' | -37.7 | NC_006635.1 | + | 24745 | 0.76 | 0.986652 |
Target: 5'- -----------aAUACGCCCUUUUGGc -3' miRNA: 3'- uuuuuuuuuuaaUAUGCGGGAGAACC- -5' |
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31422 | 3' | -37.7 | NC_006635.1 | + | 26851 | 0.81 | 0.877562 |
Target: 5'- ---------uUUAUACGCCCUUUUGGc -3' miRNA: 3'- uuuuuuuuuuAAUAUGCGGGAGAACC- -5' |
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31422 | 3' | -37.7 | NC_006635.1 | + | 24786 | 0.81 | 0.877562 |
Target: 5'- ---------uUUAUACGCCCUUUUGGc -3' miRNA: 3'- uuuuuuuuuuAAUAUGCGGGAGAACC- -5' |
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31422 | 3' | -37.7 | NC_006635.1 | + | 26882 | 0.81 | 0.868118 |
Target: 5'- ----uAAAAGUUAcACGCCCUUUUGGc -3' miRNA: 3'- uuuuuUUUUUAAUaUGCGGGAGAACC- -5' |
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31422 | 3' | -37.7 | NC_006635.1 | + | 26639 | 0.95 | 0.2758 |
Target: 5'- gAAGuuAAAAAUUAUACGCCCUUUUGGc -3' miRNA: 3'- -UUUuuUUUUUAAUAUGCGGGAGAACC- -5' |
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31422 | 3' | -37.7 | NC_006635.1 | + | 26795 | 1.11 | 0.035218 |
Target: 5'- aAAAAAAAAAAUUAUACGCCCUCUUGGc -3' miRNA: 3'- -UUUUUUUUUUAAUAUGCGGGAGAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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