miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31547 5' -46.9 NC_006820.1 + 103675 0.66 0.999969
Target:  5'- aGCGACUaagcaguuUACGCUUgguuaUACGauGAAGCGUUu -3'
miRNA:   3'- cUGCUGG--------AUGCGAA-----AUGU--UUUCGCAA- -5'
31547 5' -46.9 NC_006820.1 + 47258 0.66 0.999926
Target:  5'- --aGACCUAUGCgUUUGCAAAuGCa-- -3'
miRNA:   3'- cugCUGGAUGCG-AAAUGUUUuCGcaa -5'
31547 5' -46.9 NC_006820.1 + 146050 0.7 0.996824
Target:  5'- uGCGACUUGCGCUUaagaucUGCAAAAGacauGUUg -3'
miRNA:   3'- cUGCUGGAUGCGAA------AUGUUUUCg---CAA- -5'
31547 5' -46.9 NC_006820.1 + 164557 1.07 0.031895
Target:  5'- cGACGACCUACGCUUUACAAAAGCGUUg -3'
miRNA:   3'- -CUGCUGGAUGCGAAAUGUUUUCGCAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.