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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31562 | 5' | -45.5 | NC_006820.1 | + | 80064 | 0.66 | 0.999998 |
Target: 5'- aGAGUGCCucuUCGccaGCUUGAUUCUc -3' miRNA: 3'- cUUCAUGGucuAGUa--CGAACUAAGAc -5' |
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31562 | 5' | -45.5 | NC_006820.1 | + | 16446 | 0.67 | 0.999985 |
Target: 5'- cGAAGgugUAGAUCAUGCacaaGAUUCUGc -3' miRNA: 3'- -CUUCaugGUCUAGUACGaa--CUAAGAC- -5' |
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31562 | 5' | -45.5 | NC_006820.1 | + | 144 | 0.67 | 0.99998 |
Target: 5'- cGAAGUGCUAGGUUuugacauuuuuuGUGCcUUGGUaaacuUCUGg -3' miRNA: 3'- -CUUCAUGGUCUAG------------UACG-AACUA-----AGAC- -5' |
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31562 | 5' | -45.5 | NC_006820.1 | + | 77447 | 0.69 | 0.999799 |
Target: 5'- uGAAGUuCUGGAgcaaAUGCUUGAUUUUGc -3' miRNA: 3'- -CUUCAuGGUCUag--UACGAACUAAGAC- -5' |
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31562 | 5' | -45.5 | NC_006820.1 | + | 114026 | 1.09 | 0.034187 |
Target: 5'- uGAAGUACCAGAUCAUGCUUGAUUCUGu -3' miRNA: 3'- -CUUCAUGGUCUAGUACGAACUAAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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