Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32078 | 5' | -55.6 | NC_007016.1 | + | 78004 | 0.66 | 0.884395 |
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Target: 5'- uGGCGGCCuGgUCCCUauacgaGGAGGAg-- -3' miRNA: 3'- -UCGCCGG-CgAGGGAcaa---CCUUCUaau -5' |
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| 32078 | 5' | -55.6 | NC_007016.1 | + | 115551 | 0.66 | 0.877137 |
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Target: 5'- -aCGGCCGUaaUCCCUuaagccGGggGAUUAa -3' miRNA: 3'- ucGCCGGCG--AGGGAcaa---CCuuCUAAU- -5' |
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| 32078 | 5' | -55.6 | NC_007016.1 | + | 68477 | 0.66 | 0.869648 |
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Target: 5'- cGCGGgCGgUCCCgGgaaUGGggGGUUu -3' miRNA: 3'- uCGCCgGCgAGGGaCa--ACCuuCUAAu -5' |
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| 32078 | 5' | -55.6 | NC_007016.1 | + | 130177 | 0.69 | 0.683145 |
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Target: 5'- cGGCGGCCGCgggCCCgggccggccgGgcGGAGGGc-- -3' miRNA: 3'- -UCGCCGGCGa--GGGa---------CaaCCUUCUaau -5' |
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| 32078 | 5' | -55.6 | NC_007016.1 | + | 25381 | 0.71 | 0.567784 |
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Target: 5'- gGGCccuccGCCGCUCCCUGa-GGAGGAa-- -3' miRNA: 3'- -UCGc----CGGCGAGGGACaaCCUUCUaau -5' |
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| 32078 | 5' | -55.6 | NC_007016.1 | + | 17371 | 1.07 | 0.002893 |
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Target: 5'- gAGCGGCCGCUCCCUGUUGGAAGAUUAa -3' miRNA: 3'- -UCGCCGGCGAGGGACAACCUUCUAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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