Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32406 | 3' | -40.1 | NC_007064.1 | + | 13368 | 0.66 | 0.999996 |
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Target: 5'- uUGACGCAucaaaguucgagggGuUAGGUAAGGg--AGACa -3' miRNA: 3'- uGCUGCGU--------------UuAUUUAUUCCaaaUCUG- -5' |
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| 32406 | 3' | -40.1 | NC_007064.1 | + | 9954 | 0.67 | 0.999983 |
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Target: 5'- aACGcACGCAAGUu--UGAGGaUUGGGu -3' miRNA: 3'- -UGC-UGCGUUUAuuuAUUCCaAAUCUg -5' |
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| 32406 | 3' | -40.1 | NC_007064.1 | + | 16055 | 0.67 | 0.999983 |
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Target: 5'- -gGACGCAAAgcgcuuuuAAUAGGGguaacuaUUGGACc -3' miRNA: 3'- ugCUGCGUUUau------UUAUUCCa------AAUCUG- -5' |
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| 32406 | 3' | -40.1 | NC_007064.1 | + | 28554 | 0.73 | 0.993111 |
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Target: 5'- gUGGCGCGAGgagGAUGAGGgaUGGAa -3' miRNA: 3'- uGCUGCGUUUau-UUAUUCCaaAUCUg -5' |
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| 32406 | 3' | -40.1 | NC_007064.1 | + | 4687 | 0.73 | 0.99185 |
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Target: 5'- gACGACgGCAcuGUAAA--AGGUUUAGAUg -3' miRNA: 3'- -UGCUG-CGUu-UAUUUauUCCAAAUCUG- -5' |
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| 32406 | 3' | -40.1 | NC_007064.1 | + | 4804 | 1.13 | 0.020211 |
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Target: 5'- gACGACGCAAAUAAAUAAGGUUUAGACu -3' miRNA: 3'- -UGCUGCGUUUAUUUAUUCCAAAUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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