Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32779 | 3' | -55.7 | NC_007346.1 | + | 215429 | 0.66 | 0.986269 |
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Target: 5'- -cUGUGCCUU-GAUAUCagaccgcgaGCGGCCAAu -3' miRNA: 3'- aaGCACGGAAgCUGUAGg--------CGCUGGUU- -5' |
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| 32779 | 3' | -55.7 | NC_007346.1 | + | 274879 | 0.66 | 0.978832 |
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Target: 5'- -cCGUGCaacacgcCGACAUCCGC-ACCAu -3' miRNA: 3'- aaGCACGgaa----GCUGUAGGCGcUGGUu -5' |
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| 32779 | 3' | -55.7 | NC_007346.1 | + | 32075 | 0.67 | 0.974151 |
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Target: 5'- cUCGUGCacaUGuACAUCCGCG-CCAGa -3' miRNA: 3'- aAGCACGgaaGC-UGUAGGCGCuGGUU- -5' |
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| 32779 | 3' | -55.7 | NC_007346.1 | + | 207074 | 0.67 | 0.971547 |
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Target: 5'- aUCGcGCCacugcggUCG-CAUCCGCGACUg- -3' miRNA: 3'- aAGCaCGGa------AGCuGUAGGCGCUGGuu -5' |
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| 32779 | 3' | -55.7 | NC_007346.1 | + | 24395 | 0.7 | 0.885837 |
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Target: 5'- aUCGcGCCUUCGGCAUCauacagauguugacgCGCGcACCAu -3' miRNA: 3'- aAGCaCGGAAGCUGUAG---------------GCGC-UGGUu -5' |
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| 32779 | 3' | -55.7 | NC_007346.1 | + | 121971 | 0.75 | 0.639195 |
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Target: 5'- --aGUGCCUUCGAUuUCCGCuGCCGAu -3' miRNA: 3'- aagCACGGAAGCUGuAGGCGcUGGUU- -5' |
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| 32779 | 3' | -55.7 | NC_007346.1 | + | 171402 | 1.04 | 0.015119 |
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Target: 5'- uUUCGUGCCUUCGACAUCCGCGACCAAg -3' miRNA: 3'- -AAGCACGGAAGCUGUAGGCGCUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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