Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3306 | 5' | -53.2 | NC_001545.1 | + | 2781 | 0.66 | 0.29816 |
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Target: 5'- cCGCAGCaucGUCGCGCUaGCcgccGCGCg -3' miRNA: 3'- -GUGUCGac-UAGCGUGAcCGaaa-CGCG- -5' |
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| 3306 | 5' | -53.2 | NC_001545.1 | + | 3661 | 0.66 | 0.29816 |
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Target: 5'- gCAuCGGCgUGGcUCGCgcuccaccGCUGGCUcaGCGCg -3' miRNA: 3'- -GU-GUCG-ACU-AGCG--------UGACCGAaaCGCG- -5' |
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| 3306 | 5' | -53.2 | NC_001545.1 | + | 439 | 0.66 | 0.281152 |
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Target: 5'- gGCAcGCUGAcuacgugugCGCGCUGcGUg--GCGCa -3' miRNA: 3'- gUGU-CGACUa--------GCGUGAC-CGaaaCGCG- -5' |
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| 3306 | 5' | -53.2 | NC_001545.1 | + | 4772 | 0.67 | 0.242015 |
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Target: 5'- gACGGCUcacugCGCGCUgcggGGCUcaGCGCg -3' miRNA: 3'- gUGUCGAcua--GCGUGA----CCGAaaCGCG- -5' |
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| 3306 | 5' | -53.2 | NC_001545.1 | + | 7200 | 0.67 | 0.242015 |
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Target: 5'- gGCGGCa---CGCGCUGGCaccgacugcUGCGCa -3' miRNA: 3'- gUGUCGacuaGCGUGACCGaa-------ACGCG- -5' |
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| 3306 | 5' | -53.2 | NC_001545.1 | + | 7504 | 0.67 | 0.242015 |
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Target: 5'- gGCAGCUGAUgGUGgUGGUggccgUaaugcugcccgUGCGCa -3' miRNA: 3'- gUGUCGACUAgCGUgACCGa----A-----------ACGCG- -5' |
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| 3306 | 5' | -53.2 | NC_001545.1 | + | 7264 | 0.68 | 0.214063 |
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Target: 5'- uCGCcGUcGAggccgCGCACUGGCa-UGCGCa -3' miRNA: 3'- -GUGuCGaCUa----GCGUGACCGaaACGCG- -5' |
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| 3306 | 5' | -53.2 | NC_001545.1 | + | 2231 | 0.68 | 0.194958 |
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Target: 5'- gGCGGCggggGGUCGCcgGgUGGCggUGaCGCg -3' miRNA: 3'- gUGUCGa---CUAGCG--UgACCGaaAC-GCG- -5' |
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| 3306 | 5' | -53.2 | NC_001545.1 | + | 2309 | 0.7 | 0.146359 |
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Target: 5'- gGCGGCgGGUCGCGCgcaGGCgcgggGgGCg -3' miRNA: 3'- gUGUCGaCUAGCGUGa--CCGaaa--CgCG- -5' |
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| 3306 | 5' | -53.2 | NC_001545.1 | + | 2044 | 1.13 | 5.8e-05 |
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Target: 5'- cCACAGCUGAUCGCACUGGCUUUGCGCa -3' miRNA: 3'- -GUGUCGACUAGCGUGACCGAAACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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