Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3313 | 5' | -59.7 | NC_001545.1 | + | 494 | 0.65 | 0.137506 |
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Target: 5'- cCACCcuGAGGACgucccgcacggcgGUCGCGCCGUggCGGa -3' miRNA: 3'- -GUGG--CUCCUGa------------CGGUGCGGCGa-GUCc -5' |
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| 3313 | 5' | -59.7 | NC_001545.1 | + | 7755 | 0.66 | 0.135004 |
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Target: 5'- gCGCCGGccccCUGCCACGCCGgcCUCAa- -3' miRNA: 3'- -GUGGCUccu-GACGGUGCGGC--GAGUcc -5' |
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| 3313 | 5' | -59.7 | NC_001545.1 | + | 2689 | 0.66 | 0.12311 |
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Target: 5'- gGCCGGGuGucgccACUGCCuCGCCGgUgGGGc -3' miRNA: 3'- gUGGCUC-C-----UGACGGuGCGGCgAgUCC- -5' |
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| 3313 | 5' | -59.7 | NC_001545.1 | + | 9014 | 0.66 | 0.12311 |
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Target: 5'- aGCCGGGGGCgcucuggcgugGCCcccacaaGCCGCgagcagUCAGGg -3' miRNA: 3'- gUGGCUCCUGa----------CGGug-----CGGCG------AGUCC- -5' |
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| 3313 | 5' | -59.7 | NC_001545.1 | + | 5981 | 0.68 | 0.090155 |
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Target: 5'- uGCCGc--GCUGCgCGCGCC-CUCGGGg -3' miRNA: 3'- gUGGCuccUGACG-GUGCGGcGAGUCC- -5' |
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| 3313 | 5' | -59.7 | NC_001545.1 | + | 8214 | 0.68 | 0.084654 |
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Target: 5'- gCGgUGAGGGCggcgGCgGCGCCGCggcggcgaCAGGc -3' miRNA: 3'- -GUgGCUCCUGa---CGgUGCGGCGa-------GUCC- -5' |
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| 3313 | 5' | -59.7 | NC_001545.1 | + | 4764 | 0.69 | 0.079473 |
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Target: 5'- aGCuCGAGGACgGCuCACuGCgCGCUgCGGGg -3' miRNA: 3'- gUG-GCUCCUGaCG-GUG-CG-GCGA-GUCC- -5' |
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| 3313 | 5' | -59.7 | NC_001545.1 | + | 2743 | 0.69 | 0.079473 |
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Target: 5'- -cCCGAGGAC-GCCGCGgCGCgacggCGuGGa -3' miRNA: 3'- guGGCUCCUGaCGGUGCgGCGa----GU-CC- -5' |
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| 3313 | 5' | -59.7 | NC_001545.1 | + | 5441 | 1.11 | 2.1e-05 |
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Target: 5'- aCACCGAGGACUGCCACGCCGCUCAGGg -3' miRNA: 3'- -GUGGCUCCUGACGGUGCGGCGAGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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