Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33132 | 5' | -58.5 | NC_007497.1 | + | 40171 | 0.66 | 0.451712 |
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Target: 5'- aUGUUGGCCUCGU----CG-ACGGCAg -3' miRNA: 3'- aACAGCCGGAGCAacucGCgUGCCGU- -5' |
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| 33132 | 5' | -58.5 | NC_007497.1 | + | 43617 | 0.66 | 0.432086 |
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Target: 5'- --aUgGGCCUCGUcggggucgaUG-GUGCugGGCAg -3' miRNA: 3'- aacAgCCGGAGCA---------ACuCGCGugCCGU- -5' |
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| 33132 | 5' | -58.5 | NC_007497.1 | + | 5832 | 0.66 | 0.432086 |
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Target: 5'- -cGUCGGCUgac--GAGCgaGCGCGGCGa -3' miRNA: 3'- aaCAGCCGGagcaaCUCG--CGUGCCGU- -5' |
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| 33132 | 5' | -58.5 | NC_007497.1 | + | 11963 | 0.67 | 0.367532 |
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Target: 5'- -aG-CGGCUcaCGUUGAGCGCGauUGGCGu -3' miRNA: 3'- aaCaGCCGGa-GCAACUCGCGU--GCCGU- -5' |
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| 33132 | 5' | -58.5 | NC_007497.1 | + | 40579 | 0.72 | 0.188747 |
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Target: 5'- aUGcUCGGCUUCGU---GCGCAUGGCGc -3' miRNA: 3'- aAC-AGCCGGAGCAacuCGCGUGCCGU- -5' |
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| 33132 | 5' | -58.5 | NC_007497.1 | + | 2130 | 0.72 | 0.183691 |
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Target: 5'- gUGaCGGCCUUGguucGAGUgGCACGGCAc -3' miRNA: 3'- aACaGCCGGAGCaa--CUCG-CGUGCCGU- -5' |
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| 33132 | 5' | -58.5 | NC_007497.1 | + | 29944 | 0.72 | 0.173937 |
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Target: 5'- --uUCGGCCaUGUUGGGCGaUGCGGCGa -3' miRNA: 3'- aacAGCCGGaGCAACUCGC-GUGCCGU- -5' |
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| 33132 | 5' | -58.5 | NC_007497.1 | + | 5709 | 0.73 | 0.164647 |
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Target: 5'- -aGUCGGuCCUCagUGAuCGCACGGCAc -3' miRNA: 3'- aaCAGCC-GGAGcaACUcGCGUGCCGU- -5' |
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| 33132 | 5' | -58.5 | NC_007497.1 | + | 21607 | 0.74 | 0.143349 |
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Target: 5'- gUGcUCGGCCgCGUUgacGAGCGCGCGGgAg -3' miRNA: 3'- aAC-AGCCGGaGCAA---CUCGCGUGCCgU- -5' |
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| 33132 | 5' | -58.5 | NC_007497.1 | + | 23662 | 1.05 | 0.00055 |
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Target: 5'- gUUGUCGGCCUCGUUGAGCGCACGGCAc -3' miRNA: 3'- -AACAGCCGGAGCAACUCGCGUGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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