Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33156 | 3' | -54.1 | NC_007497.1 | + | 17686 | 0.67 | 0.610558 |
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Target: 5'- uCGGCg--GCCGuauccgcuccGCUGCCGGcGCGGUUg -3' miRNA: 3'- -GCUGuaaCGGU----------CGACGGCCaCGUUAA- -5' |
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| 33156 | 3' | -54.1 | NC_007497.1 | + | 24672 | 0.68 | 0.520076 |
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Target: 5'- cCGAaAUUGCCGGCUGCaGGcgGCAc-- -3' miRNA: 3'- -GCUgUAACGGUCGACGgCCa-CGUuaa -5' |
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| 33156 | 3' | -54.1 | NC_007497.1 | + | 35152 | 0.68 | 0.509095 |
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Target: 5'- cCGACGUcgcgGCCGGCgcGCCgGGUGUGAa- -3' miRNA: 3'- -GCUGUAa---CGGUCGa-CGG-CCACGUUaa -5' |
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| 33156 | 3' | -54.1 | NC_007497.1 | + | 1842 | 0.73 | 0.285135 |
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Target: 5'- aCGGuCGUUaCCAGCgGCCGGUGCAu-- -3' miRNA: 3'- -GCU-GUAAcGGUCGaCGGCCACGUuaa -5' |
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| 33156 | 3' | -54.1 | NC_007497.1 | + | 23224 | 0.77 | 0.146452 |
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Target: 5'- gGGCGUcGCCGGCUGCCGGgcuuccgGCGGa- -3' miRNA: 3'- gCUGUAaCGGUCGACGGCCa------CGUUaa -5' |
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| 33156 | 3' | -54.1 | NC_007497.1 | + | 31078 | 1.05 | 0.001401 |
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Target: 5'- cCGACAUUGCCAGCUGCCGGUGCAAUUc -3' miRNA: 3'- -GCUGUAACGGUCGACGGCCACGUUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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