miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33220 5' -66.8 NC_007523.1 + 4570 0.66 0.019672
Target:  5'- aGCGCCCGUGUguaggCGUugagugggcagagauGGUCCUgcgaCGCCCCc -3'
miRNA:   3'- aCGCGGGUACG-----GCG---------------CCGGGG----GUGGGG- -5'
33220 5' -66.8 NC_007523.1 + 491 0.66 0.018779
Target:  5'- uUGCcuGUCCGUGUCGUGGCgCUCGggacucucgacuCCCCu -3'
miRNA:   3'- -ACG--CGGGUACGGCGCCGgGGGU------------GGGG- -5'
33220 5' -66.8 NC_007523.1 + 1951 0.68 0.012187
Target:  5'- cGCGCCCGUGCauacgGCgaGGCCaaCUCGCCUg -3'
miRNA:   3'- aCGCGGGUACGg----CG--CCGG--GGGUGGGg -5'
33220 5' -66.8 NC_007523.1 + 2190 0.74 0.003426
Target:  5'- gGCGCUgGUGUaaugggaggcggCGUGGCCCCCccuCCCCc -3'
miRNA:   3'- aCGCGGgUACG------------GCGCCGGGGGu--GGGG- -5'
33220 5' -66.8 NC_007523.1 + 1530 1.1 0.000001
Target:  5'- cUGCGCCCAUGCCGCGGCCCCCACCCCu -3'
miRNA:   3'- -ACGCGGGUACGGCGCCGGGGGUGGGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.