Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33266 | 3' | -52.9 | NC_007605.1 | + | 147245 | 0.66 | 0.972547 |
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Target: 5'- -uGGUaagGGGGCugGUGCggacgccUGUGUUUAGu -3' miRNA: 3'- uuCUAa--CCCCGugUACG-------ACGCAAGUC- -5' |
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| 33266 | 3' | -52.9 | NC_007605.1 | + | 38383 | 0.68 | 0.932963 |
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Target: 5'- cGGGUUGGuGGCAC-UGUUGCGccCGGc -3' miRNA: 3'- uUCUAACC-CCGUGuACGACGCaaGUC- -5' |
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| 33266 | 3' | -52.9 | NC_007605.1 | + | 149534 | 0.69 | 0.897017 |
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Target: 5'- uAGAaaugGGGGCGUGUGCUGCGccagCAGg -3' miRNA: 3'- uUCUaa--CCCCGUGUACGACGCaa--GUC- -5' |
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| 33266 | 3' | -52.9 | NC_007605.1 | + | 122314 | 0.69 | 0.868082 |
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Target: 5'- cGGGAUUcGGGGC-CAgcUGCUGCGUaacgUCAu -3' miRNA: 3'- -UUCUAA-CCCCGuGU--ACGACGCA----AGUc -5' |
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| 33266 | 3' | -52.9 | NC_007605.1 | + | 8322 | 0.75 | 0.584321 |
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Target: 5'- cAAGAUaGGGGCGCGauUGCUGCGaUCuGg -3' miRNA: 3'- -UUCUAaCCCCGUGU--ACGACGCaAGuC- -5' |
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| 33266 | 3' | -52.9 | NC_007605.1 | + | 140535 | 0.8 | 0.324003 |
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Target: 5'- gGAGAggcggGGGGUAUGUGCUGCGUUgGGa -3' miRNA: 3'- -UUCUaa---CCCCGUGUACGACGCAAgUC- -5' |
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| 33266 | 3' | -52.9 | NC_007605.1 | + | 4123 | 1.06 | 0.007955 |
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Target: 5'- cAAGAUUGGGGCACAUGCUGCGUUCAGc -3' miRNA: 3'- -UUCUAACCCCGUGUACGACGCAAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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