miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
3489 3' -40.9 NC_001611.1 + 7048 0.66 1
Target:  5'- aUGUcUUCUAAAGGagguagugGCGGcauguGGAGUGUCUu -3'
miRNA:   3'- -ACA-AAGAUUUCU--------UGCC-----UCUUAUAGAu -5'
3489 3' -40.9 NC_001611.1 + 34120 0.67 1
Target:  5'- cGUcaUUCUAAAaGACGGAGGAUcuccAUCa- -3'
miRNA:   3'- aCA--AAGAUUUcUUGCCUCUUA----UAGau -5'
3489 3' -40.9 NC_001611.1 + 119011 0.67 1
Target:  5'- --aUUCUuugguAGAAUGGGGAAcAUCUAc -3'
miRNA:   3'- acaAAGAuu---UCUUGCCUCUUaUAGAU- -5'
3489 3' -40.9 NC_001611.1 + 105272 0.66 1
Target:  5'- ---aUCgaAAAGAACGGAGAAUuguuGUCg- -3'
miRNA:   3'- acaaAGa-UUUCUUGCCUCUUA----UAGau -5'
3489 3' -40.9 NC_001611.1 + 45057 0.77 0.997724
Target:  5'- aUGUUgUCUGAAGGACGGAuGAAU-UCUAa -3'
miRNA:   3'- -ACAA-AGAUUUCUUGCCU-CUUAuAGAU- -5'
3489 3' -40.9 NC_001611.1 + 44807 1.09 0.105466
Target:  5'- cUGUUUCUAAAGAACGGAGAAUAUCUAu -3'
miRNA:   3'- -ACAAAGAUUUCUUGCCUCUUAUAGAU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.