Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4264 | 5' | -63.7 | NC_001710.1 | + | 6530 | 0.66 | 0.071322 |
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Target: 5'- gACCUC-CGCCUAuaagcugcUGCGCCAGCaaaUCCUa -3' miRNA: 3'- gUGGGGcGUGGGU--------GCGCGGUCG---AGGG- -5' |
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| 4264 | 5' | -63.7 | NC_001710.1 | + | 2338 | 0.66 | 0.062948 |
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Target: 5'- gGCCCUGCccugUCCugGUGUCugGGaCUCCCg -3' miRNA: 3'- gUGGGGCGu---GGGugCGCGG--UC-GAGGG- -5' |
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| 4264 | 5' | -63.7 | NC_001710.1 | + | 6944 | 0.67 | 0.04896 |
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Target: 5'- -cCCCCGUACuCCAU-CGCCAGCacuuaUCUCg -3' miRNA: 3'- guGGGGCGUG-GGUGcGCGGUCG-----AGGG- -5' |
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| 4264 | 5' | -63.7 | NC_001710.1 | + | 2546 | 0.67 | 0.04896 |
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Target: 5'- gGCCCCgagcacugagguGCGCCCGCGgguCGCCGaaaUCCCc -3' miRNA: 3'- gUGGGG------------CGUGGGUGC---GCGGUcg-AGGG- -5' |
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| 4264 | 5' | -63.7 | NC_001710.1 | + | 2159 | 0.71 | 0.025952 |
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Target: 5'- uCAUCCCGC-CCC-CGCGCUgguuGCUCUUg -3' miRNA: 3'- -GUGGGGCGuGGGuGCGCGGu---CGAGGG- -5' |
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| 4264 | 5' | -63.7 | NC_001710.1 | + | 4184 | 1.1 | 0.000007 |
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Target: 5'- gCACCCCGCACCCACGCGCCAGCUCCCg -3' miRNA: 3'- -GUGGGGCGUGGGUGCGCGGUCGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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