Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4327 | 5' | -57.1 | NC_001720.1 | + | 2351 | 0.66 | 0.556261 |
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Target: 5'- --gGCACCCCUAUGuccGGAGCuCCg- -3' miRNA: 3'- gagCGUGGGGAUGCuacCCUUG-GGau -5' |
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| 4327 | 5' | -57.1 | NC_001720.1 | + | 22681 | 0.66 | 0.545388 |
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Target: 5'- -gUGCGCCCCUccgggcACGAacuUGGGcuccaGACCCg- -3' miRNA: 3'- gaGCGUGGGGA------UGCU---ACCC-----UUGGGau -5' |
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| 4327 | 5' | -57.1 | NC_001720.1 | + | 3492 | 0.66 | 0.534584 |
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Target: 5'- aUCGaGCCCCUguucacGCGcGUGGGcauuACCCUAa -3' miRNA: 3'- gAGCgUGGGGA------UGC-UACCCu---UGGGAU- -5' |
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| 4327 | 5' | -57.1 | NC_001720.1 | + | 17221 | 0.66 | 0.51746 |
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Target: 5'- --gGCACCCCcgcgaccgcgagggaUcucGCGAcGGGAACCCUc -3' miRNA: 3'- gagCGUGGGG---------------A---UGCUaCCCUUGGGAu -5' |
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| 4327 | 5' | -57.1 | NC_001720.1 | + | 6655 | 1.06 | 0.000706 |
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Target: 5'- cCUCGCACCCCUACGAUGGGAACCCUAc -3' miRNA: 3'- -GAGCGUGGGGAUGCUACCCUUGGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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