Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 447 | 5' | -51.1 | AC_000013.1 | + | 12590 | 0.66 | 0.857883 |
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Target: 5'- gCGCAGCAGCgagCGCGGcgUCCcCGUc--- -3' miRNA: 3'- -GUGUUGUCGa--GCGCU--AGGcGCAuuau -5' |
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| 447 | 5' | -51.1 | AC_000013.1 | + | 13803 | 0.67 | 0.820869 |
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Target: 5'- gCGCAACAGCUgccaccaaaCGCG-UuuGCGUAc-- -3' miRNA: 3'- -GUGUUGUCGA---------GCGCuAggCGCAUuau -5' |
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| 447 | 5' | -51.1 | AC_000013.1 | + | 25335 | 0.68 | 0.758983 |
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Target: 5'- gGCAGCAGaC-CGCGAUCUuuGgGUAGUGa -3' miRNA: 3'- gUGUUGUC-GaGCGCUAGG--CgCAUUAU- -5' |
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| 447 | 5' | -51.1 | AC_000013.1 | + | 11839 | 0.69 | 0.714663 |
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Target: 5'- uCGgAACAGCUCGUG-UCUGCGg---- -3' miRNA: 3'- -GUgUUGUCGAGCGCuAGGCGCauuau -5' |
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| 447 | 5' | -51.1 | AC_000013.1 | + | 14863 | 0.72 | 0.497601 |
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Target: 5'- aGCGACGcGUUCGCGAUCuCGCGg---- -3' miRNA: 3'- gUGUUGU-CGAGCGCUAG-GCGCauuau -5' |
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| 447 | 5' | -51.1 | AC_000013.1 | + | 1647 | 1.07 | 0.002416 |
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Target: 5'- cCACAACAGCUCGCGAUCCGCGUAAUAg -3' miRNA: 3'- -GUGUUGUCGAGCGCUAGGCGCAUUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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