Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 460 | 5' | -61.1 | AC_000013.1 | + | 19175 | 0.66 | 0.40912 |
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Target: 5'- cGGUGG-UugGGCCCUgCGaGACGGa -3' miRNA: 3'- cCCGCCaGugCCGGGAgGUgCUGCUg -5' |
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| 460 | 5' | -61.1 | AC_000013.1 | + | 8217 | 0.66 | 0.40912 |
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Target: 5'- uGGGaUGGUCGCGcGCCUUCUuCaGCGAg -3' miRNA: 3'- -CCC-GCCAGUGC-CGGGAGGuGcUGCUg -5' |
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| 460 | 5' | -61.1 | AC_000013.1 | + | 18170 | 0.67 | 0.349353 |
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Target: 5'- gGGGCGGgaACGGCgUCUgCugGACcGACc -3' miRNA: 3'- -CCCGCCagUGCCG-GGAgGugCUG-CUG- -5' |
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| 460 | 5' | -61.1 | AC_000013.1 | + | 7492 | 0.67 | 0.325712 |
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Target: 5'- cGGCGGccgCACGGaUCgCUCC-CGAgGACg -3' miRNA: 3'- cCCGCCa--GUGCC-GG-GAGGuGCUgCUG- -5' |
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| 460 | 5' | -61.1 | AC_000013.1 | + | 13669 | 0.68 | 0.303281 |
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Target: 5'- cGG-GGUCGCGuGCUgaCCGcCGACGACu -3' miRNA: 3'- cCCgCCAGUGC-CGGgaGGU-GCUGCUG- -5' |
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| 460 | 5' | -61.1 | AC_000013.1 | + | 12231 | 0.68 | 0.296072 |
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Target: 5'- cGGGCGG-CGCcaucGUCCUCCGCaACaGACg -3' miRNA: 3'- -CCCGCCaGUGc---CGGGAGGUGcUG-CUG- -5' |
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| 460 | 5' | -61.1 | AC_000013.1 | + | 13439 | 0.69 | 0.262027 |
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Target: 5'- aGGGCGGaCGCGGU--UUCGCGuCGGCg -3' miRNA: 3'- -CCCGCCaGUGCCGggAGGUGCuGCUG- -5' |
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| 460 | 5' | -61.1 | AC_000013.1 | + | 19618 | 0.71 | 0.188356 |
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Target: 5'- aGGaGCGGUagugggaACGGauuCCC-CCGCGGCGACu -3' miRNA: 3'- -CC-CGCCAg------UGCC---GGGaGGUGCUGCUG- -5' |
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| 460 | 5' | -61.1 | AC_000013.1 | + | 8926 | 1.11 | 0.000176 |
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Target: 5'- uGGGCGGUCACGGCCCUCCACGACGACa -3' miRNA: 3'- -CCCGCCAGUGCCGGGAGGUGCUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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