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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6024 | 3' | -57.3 | NC_001809.1 | + | 6661 | 0.67 | 0.144636 |
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Target: 5'- aCCCaGGGUCGCCAg-ACCCAGAa-- -3' miRNA: 3'- aGGGaCUCGGUGGUagUGGGUCUgug -5' |
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| 6024 | 3' | -57.3 | NC_001809.1 | + | 7199 | 0.68 | 0.131706 |
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Target: 5'- gUCCCUGguuggGGCCACCccCACCUcacugauaguGGGCAUu -3' miRNA: 3'- -AGGGAC-----UCGGUGGuaGUGGG----------UCUGUG- -5' |
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| 6024 | 3' | -57.3 | NC_001809.1 | + | 6489 | 0.68 | 0.121372 |
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Target: 5'- aUUCCUGucagaacaucuccgaAGCUGCgA-CGCCCAGACGCg -3' miRNA: 3'- -AGGGAC---------------UCGGUGgUaGUGGGUCUGUG- -5' |
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| 6024 | 3' | -57.3 | NC_001809.1 | + | 6301 | 0.68 | 0.116124 |
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Target: 5'- cUCCCaUGuccAGCUucgguCCGUCACgCUAGACACg -3' miRNA: 3'- -AGGG-AC---UCGGu----GGUAGUG-GGUCUGUG- -5' |
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| 6024 | 3' | -57.3 | NC_001809.1 | + | 10788 | 1.1 | 0.00004 |
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Target: 5'- cUCCCUGAGCCACCAUCACCCAGACACa -3' miRNA: 3'- -AGGGACUCGGUGGUAGUGGGUCUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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