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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6105 | 5' | -53.1 | NC_001826.1 | + | 97965 | 0.66 | 0.95159 |
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Target: 5'- ---gCGCUCAACAGGCUCUgcUCCAUc -3' miRNA: 3'- gccgGUGAGUUGUCUGAGAa-GGGUAc -5' |
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| 6105 | 5' | -53.1 | NC_001826.1 | + | 109660 | 0.66 | 0.947223 |
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Target: 5'- uGGUgACUCAGCAGACa--UCgCAUGu -3' miRNA: 3'- gCCGgUGAGUUGUCUGagaAGgGUAC- -5' |
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| 6105 | 5' | -53.1 | NC_001826.1 | + | 12209 | 0.66 | 0.939711 |
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Target: 5'- gGGCCACUgGACauaguucuggaugggAGACUCcUCCUg-- -3' miRNA: 3'- gCCGGUGAgUUG---------------UCUGAGaAGGGuac -5' |
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| 6105 | 5' | -53.1 | NC_001826.1 | + | 82045 | 0.67 | 0.927211 |
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Target: 5'- gGGCC-UUCAggacagcauGCAGGUUCUUUCCAUGa -3' miRNA: 3'- gCCGGuGAGU---------UGUCUGAGAAGGGUAC- -5' |
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| 6105 | 5' | -53.1 | NC_001826.1 | + | 117370 | 0.67 | 0.915657 |
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Target: 5'- gGGCUcugacGCggaGAcCGGACUCUUCCCAa- -3' miRNA: 3'- gCCGG-----UGag-UU-GUCUGAGAAGGGUac -5' |
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| 6105 | 5' | -53.1 | NC_001826.1 | + | 118493 | 0.69 | 0.843012 |
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Target: 5'- uGGCC-CUCAGCAGGgUCcgggugCCUGUGg -3' miRNA: 3'- gCCGGuGAGUUGUCUgAGaa----GGGUAC- -5' |
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| 6105 | 5' | -53.1 | NC_001826.1 | + | 6566 | 0.72 | 0.677679 |
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Target: 5'- aGGCCcacCUCAACAGGCUaagaugaacaCUUgCCCAUGc -3' miRNA: 3'- gCCGGu--GAGUUGUCUGA----------GAA-GGGUAC- -5' |
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| 6105 | 5' | -53.1 | NC_001826.1 | + | 3888 | 1.08 | 0.004628 |
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Target: 5'- gCGGCCACUCAACAGACUCUUCCCAUGc -3' miRNA: 3'- -GCCGGUGAGUUGUCUGAGAAGGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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