Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 613 | 5' | -49.5 | AC_000017.1 | + | 10239 | 0.67 | 0.840958 |
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Target: 5'- cGGUCUG--GUGGcCCGGCUGCGagaGCu -3' miRNA: 3'- -CCAGGCaaUACC-GGUUGACGUgaaUG- -5' |
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| 613 | 5' | -49.5 | AC_000017.1 | + | 24503 | 0.67 | 0.821571 |
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Target: 5'- -aUCCGcUAUGGCgAggggcacaucGCUGCGCUcuUGCa -3' miRNA: 3'- ccAGGCaAUACCGgU----------UGACGUGA--AUG- -5' |
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| 613 | 5' | -49.5 | AC_000017.1 | + | 26306 | 0.69 | 0.724387 |
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Target: 5'- cGUCCGUggGUGGCggcggCAGCUGCAgCUUc- -3' miRNA: 3'- cCAGGCAa-UACCG-----GUUGACGU-GAAug -5' |
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| 613 | 5' | -49.5 | AC_000017.1 | + | 23305 | 0.69 | 0.712863 |
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Target: 5'- cGGgcugCgCGUUGUGGCUGcaccGCUGCGCUg-- -3' miRNA: 3'- -CCa---G-GCAAUACCGGU----UGACGUGAaug -5' |
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| 613 | 5' | -49.5 | AC_000017.1 | + | 10119 | 0.7 | 0.67779 |
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Target: 5'- cGG-CUaaUAUGGCCuGCUGCACcUGCg -3' miRNA: 3'- -CCaGGcaAUACCGGuUGACGUGaAUG- -5' |
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| 613 | 5' | -49.5 | AC_000017.1 | + | 26827 | 0.73 | 0.513512 |
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Target: 5'- -uUCUG-UGUGGCC-GCUGCugUUGCu -3' miRNA: 3'- ccAGGCaAUACCGGuUGACGugAAUG- -5' |
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| 613 | 5' | -49.5 | AC_000017.1 | + | 10268 | 1.14 | 0.001056 |
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Target: 5'- uGGUCCGUUAUGGCCAACUGCACUUACa -3' miRNA: 3'- -CCAGGCAAUACCGGUUGACGUGAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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