Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 669 | 3' | -65.1 | AC_000018.1 | + | 16096 | 0.66 | 0.20628 |
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Target: 5'- -aGGCGCcacaGGGCUACUCCC-GCCaugCGAg -3' miRNA: 3'- gcCCGCG----CUCGGUGAGGGuCGGg--GCU- -5' |
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| 669 | 3' | -65.1 | AC_000018.1 | + | 8504 | 0.67 | 0.154696 |
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Target: 5'- cCGGGuCcCGAGCCGC-CCCuGCcgCCCGGu -3' miRNA: 3'- -GCCC-GcGCUCGGUGaGGGuCG--GGGCU- -5' |
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| 669 | 3' | -65.1 | AC_000018.1 | + | 16297 | 0.68 | 0.146673 |
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Target: 5'- uGGGUGCGugauGCCACUaCCGGCCagCGc -3' miRNA: 3'- gCCCGCGCu---CGGUGAgGGUCGGg-GCu -5' |
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| 669 | 3' | -65.1 | AC_000018.1 | + | 17181 | 0.68 | 0.142805 |
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Target: 5'- aCGGGCGCGGGCa--UCCagGGUUCCGu -3' miRNA: 3'- -GCCCGCGCUCGgugAGGg-UCGGGGCu -5' |
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| 669 | 3' | -65.1 | AC_000018.1 | + | 11409 | 0.69 | 0.115078 |
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Target: 5'- gCGGGuCGCGAGCUGCgCCaCGGUCUgGAu -3' miRNA: 3'- -GCCC-GCGCUCGGUGaGG-GUCGGGgCU- -5' |
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| 669 | 3' | -65.1 | AC_000018.1 | + | 10693 | 1.08 | 0.000074 |
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Target: 5'- aCGGGCGCGAGCCACUCCCAGCCCCGAg -3' miRNA: 3'- -GCCCGCGCUCGGUGAGGGUCGGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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