Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6696 | 5' | -63.1 | NC_001866.1 | + | 866 | 0.66 | 0.016508 |
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Target: 5'- cUGGCGGgGGUGGGaGAGCaGCagggCCAg -3' miRNA: 3'- aACCGCCgCCGCCUcUUCGgCGa---GGU- -5' |
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| 6696 | 5' | -63.1 | NC_001866.1 | + | 2321 | 0.66 | 0.016508 |
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Target: 5'- -gGGCGG-GGCGGcccgcGggGCgCGCUCg- -3' miRNA: 3'- aaCCGCCgCCGCCu----CuuCG-GCGAGgu -5' |
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| 6696 | 5' | -63.1 | NC_001866.1 | + | 2086 | 0.67 | 0.014311 |
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Target: 5'- -aGGUGGUGuCGGAGAAGCuCGCcaccuaCCAa -3' miRNA: 3'- aaCCGCCGCcGCCUCUUCG-GCGa-----GGU- -5' |
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| 6696 | 5' | -63.1 | NC_001866.1 | + | 2208 | 0.67 | 0.013325 |
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Target: 5'- -cGGCGGggcCGGCGGgaGGAGGCgGCggccCCGa -3' miRNA: 3'- aaCCGCC---GCCGCC--UCUUCGgCGa---GGU- -5' |
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| 6696 | 5' | -63.1 | NC_001866.1 | + | 1900 | 0.68 | 0.012405 |
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Target: 5'- -cGGCGGCugGGCGaGAGggGCgGCa--- -3' miRNA: 3'- aaCCGCCG--CCGC-CUCuuCGgCGaggu -5' |
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| 6696 | 5' | -63.1 | NC_001866.1 | + | 1792 | 0.68 | 0.010374 |
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Target: 5'- cUGGCGGCGcagcaGCGGGGcagcGAGCUGCagCCu -3' miRNA: 3'- aACCGCCGC-----CGCCUC----UUCGGCGa-GGu -5' |
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| 6696 | 5' | -63.1 | NC_001866.1 | + | 1946 | 0.7 | 0.006512 |
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Target: 5'- cUGGuCGGCGGUGGAagggaagcagGAGGCgGCggCCAg -3' miRNA: 3'- aACC-GCCGCCGCCU----------CUUCGgCGa-GGU- -5' |
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| 6696 | 5' | -63.1 | NC_001866.1 | + | 2154 | 0.72 | 0.004388 |
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Target: 5'- -aGGCGGCGGCGGGGcccCC-CUCCu -3' miRNA: 3'- aaCCGCCGCCGCCUCuucGGcGAGGu -5' |
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| 6696 | 5' | -63.1 | NC_001866.1 | + | 2081 | 1.05 | 0.000003 |
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Target: 5'- cUUGGCGGCGGCGGAGAAGCCGCUCCAc -3' miRNA: 3'- -AACCGCCGCCGCCUCUUCGGCGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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