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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 7609 | 5' | -50.4 | NC_001963.1 | + | 2573 | 0.66 | 0.287049 |
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Target: 5'- gCGGGCccgucaGGCUGGGCgGgGCCGGGugCUa -3' miRNA: 3'- aGUCCG------UUGACCUGgU-UGGUUUugGG- -5' |
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| 7609 | 5' | -50.4 | NC_001963.1 | + | 2449 | 0.66 | 0.287049 |
|
Target: 5'- -gGGGgGGCgGGAUCAcGCCGccGCCCu -3' miRNA: 3'- agUCCgUUGaCCUGGU-UGGUuuUGGG- -5' |
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| 7609 | 5' | -50.4 | NC_001963.1 | + | 789 | 0.67 | 0.22947 |
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Target: 5'- gUCGGGguAUgaagacGGaACCGGCCAAcgaauugaccGGCCCg -3' miRNA: 3'- -AGUCCguUGa-----CC-UGGUUGGUU----------UUGGG- -5' |
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| 7609 | 5' | -50.4 | NC_001963.1 | + | 107 | 0.67 | 0.237056 |
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Target: 5'- -uGGGCggUggaaauaggUGGcAUCAACCGAAACCa -3' miRNA: 3'- agUCCGuuG---------ACC-UGGUUGGUUUUGGg -5' |
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| 7609 | 5' | -50.4 | NC_001963.1 | + | 5119 | 0.71 | 0.124376 |
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Target: 5'- -gGGGCAACauacuagUGGGCCcGCUggGGCCa -3' miRNA: 3'- agUCCGUUG-------ACCUGGuUGGuuUUGGg -5' |
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| 7609 | 5' | -50.4 | NC_001963.1 | + | 4324 | 1.13 | 0.000046 |
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Target: 5'- cUCAGGCAACUGGACCAACCAAAACCCu -3' miRNA: 3'- -AGUCCGUUGACCUGGUUGGUUUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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