Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7664 | 5' | -57.4 | NC_001973.1 | + | 61153 | 0.66 | 0.88271 |
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Target: 5'- aCCGGcGCCGCuCCGcGucGGGCGGUCGc -3' miRNA: 3'- aGGCUcUGGUG-GGC-Cu-UCUGCCAGUu -5' |
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| 7664 | 5' | -57.4 | NC_001973.1 | + | 45148 | 0.66 | 0.875702 |
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Target: 5'- uUCCGAGACaCGuCCaCGGGcgcgccgagcAGcCGGUCGAa -3' miRNA: 3'- -AGGCUCUG-GU-GG-GCCU----------UCuGCCAGUU- -5' |
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| 7664 | 5' | -57.4 | NC_001973.1 | + | 3893 | 0.66 | 0.875702 |
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Target: 5'- aCCGAGGCgucgcucugCGCCgGGuAGACGGgcgCGAa -3' miRNA: 3'- aGGCUCUG---------GUGGgCCuUCUGCCa--GUU- -5' |
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| 7664 | 5' | -57.4 | NC_001973.1 | + | 118957 | 0.66 | 0.861057 |
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Target: 5'- gUCgGAGguguACgGCCCGGAcaAGAaaaCGGUCAAg -3' miRNA: 3'- -AGgCUC----UGgUGGGCCU--UCU---GCCAGUU- -5' |
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| 7664 | 5' | -57.4 | NC_001973.1 | + | 23850 | 0.66 | 0.845607 |
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Target: 5'- cCCGAGcugugcgagcuGCUGuCCCGcAAGGCGGUCAAu -3' miRNA: 3'- aGGCUC-----------UGGU-GGGCcUUCUGCCAGUU- -5' |
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| 7664 | 5' | -57.4 | NC_001973.1 | + | 67831 | 0.67 | 0.79496 |
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Target: 5'- aCCGAGucgcugcucugGCCcacGCUCGG-AGACGGUCGu -3' miRNA: 3'- aGGCUC-----------UGG---UGGGCCuUCUGCCAGUu -5' |
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| 7664 | 5' | -57.4 | NC_001973.1 | + | 12064 | 0.67 | 0.79496 |
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Target: 5'- gCCGAucGACgUugCCGGAcGACGGUCc- -3' miRNA: 3'- aGGCU--CUG-GugGGCCUuCUGCCAGuu -5' |
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| 7664 | 5' | -57.4 | NC_001973.1 | + | 107316 | 0.69 | 0.690122 |
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Target: 5'- cCCGAugcuGGCgCGCCUGGAAGGCGGa--- -3' miRNA: 3'- aGGCU----CUG-GUGGGCCUUCUGCCaguu -5' |
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| 7664 | 5' | -57.4 | NC_001973.1 | + | 138726 | 1.06 | 0.003569 |
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Target: 5'- aUCCGAGACCACCCGGAAGACGGUCAAc -3' miRNA: 3'- -AGGCUCUGGUGGGCCUUCUGCCAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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