Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 772 | 3' | -46.3 | NC_000852.3 | + | 43136 | 0.66 | 1 |
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Target: 5'- -cCCGCCCCUUcGUGUgGCGAAa--- -3' miRNA: 3'- caGGUGGGGAAcUAUAaCGUUUaugc -5' |
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| 772 | 3' | -46.3 | NC_000852.3 | + | 57653 | 0.66 | 1 |
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Target: 5'- uUCCGCCCCc-GGUggUGCAucAUAUGu -3' miRNA: 3'- cAGGUGGGGaaCUAuaACGUu-UAUGC- -5' |
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| 772 | 3' | -46.3 | NC_000852.3 | + | 127593 | 0.66 | 1 |
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Target: 5'- aUUUACCCCcUGAUAcaaGCAAcgACGa -3' miRNA: 3'- cAGGUGGGGaACUAUaa-CGUUuaUGC- -5' |
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| 772 | 3' | -46.3 | NC_000852.3 | + | 102252 | 0.66 | 1 |
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Target: 5'- uUUCGgCCCUUGAUggUGCAAAg--- -3' miRNA: 3'- cAGGUgGGGAACUAuaACGUUUaugc -5' |
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| 772 | 3' | -46.3 | NC_000852.3 | + | 54110 | 0.66 | 1 |
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Target: 5'- ---uGCUCUUUGGUgaAUUGCAAAUACa -3' miRNA: 3'- caggUGGGGAACUA--UAACGUUUAUGc -5' |
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| 772 | 3' | -46.3 | NC_000852.3 | + | 196044 | 0.67 | 0.999999 |
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Target: 5'- gGUCCugUCCUUGAUGaaGCA---ACGu -3' miRNA: 3'- -CAGGugGGGAACUAUaaCGUuuaUGC- -5' |
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| 772 | 3' | -46.3 | NC_000852.3 | + | 33763 | 0.68 | 0.999991 |
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Target: 5'- -aCCACCCCaUGGUcGUUGCGAc-ACGc -3' miRNA: 3'- caGGUGGGGaACUA-UAACGUUuaUGC- -5' |
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| 772 | 3' | -46.3 | NC_000852.3 | + | 7653 | 0.68 | 0.999983 |
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Target: 5'- uUUCACUUCgUGGUAUUGCA-AUGCGa -3' miRNA: 3'- cAGGUGGGGaACUAUAACGUuUAUGC- -5' |
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| 772 | 3' | -46.3 | NC_000852.3 | + | 47128 | 0.69 | 0.999957 |
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Target: 5'- uUCCAggggcuugguaUCCCgaagaacucaagUGAUAUUGCAAGUGCa -3' miRNA: 3'- cAGGU-----------GGGGa-----------ACUAUAACGUUUAUGc -5' |
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| 772 | 3' | -46.3 | NC_000852.3 | + | 33369 | 1.1 | 0.043665 |
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Target: 5'- aGUCCACCCCUUGAUAUUGCAAAUACGu -3' miRNA: 3'- -CAGGUGGGGAACUAUAACGUUUAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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