miRNA: 3'- uuGCACCUGCGCCugG-GCaCCaaCUa -5'
miRNA: 3'- uUGC-ACCUGCGCCUGGGCAccaACUa -5'
miRNA: 3'- uUGcACCUGCGCCUGGGCA-CCaaCUa -5'
miRNA: 3'- -UUGCaCCUGCGCc-UGGGCACCa--------ACUA- -5'
miRNA: 3'- uUGCACCUGCGcCUGGGCa-CCaACUa -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8062 | 3' | -57.7 | NC_001973.1 | + | 152328 | 0.66 | 0.816138 |
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Target: 5'- -uCGUGGACGCGGugCgCG-GGcgGGg -3' miRNA: 3'- uuGCACCUGCGCCugG-GCaCCaaCUa -5' |
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| 8062 | 3' | -57.7 | NC_001973.1 | + | 81096 | 0.67 | 0.761859 |
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Target: 5'- cGCGcUGGGCGCGGGCUCGU---UGAa -3' miRNA: 3'- uUGC-ACCUGCGCCUGGGCAccaACUa -5' |
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| 8062 | 3' | -57.7 | NC_001973.1 | + | 124264 | 0.7 | 0.643005 |
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Target: 5'- uGCaUGGACGUGGGCCCGUcGGccGAc -3' miRNA: 3'- uUGcACCUGCGCCUGGGCA-CCaaCUa -5' |
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| 8062 | 3' | -57.7 | NC_001973.1 | + | 111260 | 0.7 | 0.622682 |
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Target: 5'- cGACGcGGACGCGcuGCCCGUGGgcaacucggUGGUg -3' miRNA: 3'- -UUGCaCCUGCGCc-UGGGCACCa--------ACUA- -5' |
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| 8062 | 3' | -57.7 | NC_001973.1 | + | 115379 | 0.71 | 0.562114 |
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Target: 5'- uACGUGGACGC-GACCCGccGGcUGAg -3' miRNA: 3'- uUGCACCUGCGcCUGGGCa-CCaACUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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