Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9429 | 5' | -60.3 | NC_002531.1 | + | 57944 | 0.67 | 0.628534 |
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Target: 5'- cUGGCCGucCAGCGGCgCGUggCGGuGUCCGu -3' miRNA: 3'- cACCGGCc-GUUGCCG-GUA--GUC-CAGGC- -5' |
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| 9429 | 5' | -60.3 | NC_002531.1 | + | 113355 | 0.67 | 0.618468 |
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Target: 5'- -gGGaCCGGCGAgGGCCAaucgCAGG-CUa -3' miRNA: 3'- caCC-GGCCGUUgCCGGUa---GUCCaGGc -5' |
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| 9429 | 5' | -60.3 | NC_002531.1 | + | 44161 | 0.67 | 0.607407 |
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Target: 5'- uUGGUCcGCAGCGGCCG-CAGGgaugucuUCUGg -3' miRNA: 3'- cACCGGcCGUUGCCGGUaGUCC-------AGGC- -5' |
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| 9429 | 5' | -60.3 | NC_002531.1 | + | 80824 | 0.68 | 0.558522 |
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Target: 5'- -aGGCCGG-AGCGGCC--CAGGaCCa -3' miRNA: 3'- caCCGGCCgUUGCCGGuaGUCCaGGc -5' |
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| 9429 | 5' | -60.3 | NC_002531.1 | + | 10750 | 0.69 | 0.509852 |
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Target: 5'- aUGGUgGGCAugGGCC-UC-GGcCCGa -3' miRNA: 3'- cACCGgCCGUugCCGGuAGuCCaGGC- -5' |
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| 9429 | 5' | -60.3 | NC_002531.1 | + | 83047 | 0.7 | 0.418596 |
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Target: 5'- -cGGUCcGUGAUGGCCGcCAGGUCCa -3' miRNA: 3'- caCCGGcCGUUGCCGGUaGUCCAGGc -5' |
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| 9429 | 5' | -60.3 | NC_002531.1 | + | 121473 | 0.71 | 0.401554 |
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Target: 5'- cGUGGCCGGCAucuguucggaugACGGCCggCAccuGG-CCa -3' miRNA: 3'- -CACCGGCCGU------------UGCCGGuaGU---CCaGGc -5' |
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| 9429 | 5' | -60.3 | NC_002531.1 | + | 121301 | 0.72 | 0.34551 |
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Target: 5'- cGUGGCCaGGUGcCGGCCGUCA--UCCGa -3' miRNA: 3'- -CACCGG-CCGUuGCCGGUAGUccAGGC- -5' |
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| 9429 | 5' | -60.3 | NC_002531.1 | + | 36157 | 1.09 | 0.001 |
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Target: 5'- cGUGGCCGGCAACGGCCAUCAGGUCCGa -3' miRNA: 3'- -CACCGGCCGUUGCCGGUAGUCCAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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