Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9521 | 3' | -52.9 | NC_002532.2 | + | 1867 | 0.66 | 0.35529 |
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Target: 5'- uGCCAUuggguUGAUACCCAguGugGGCa--- -3' miRNA: 3'- uCGGUGu----ACUAUGGGU--UugCCGacgu -5' |
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| 9521 | 3' | -52.9 | NC_002532.2 | + | 10937 | 0.68 | 0.27523 |
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Target: 5'- cAGCCAaaAUGGaacACCCAAACgcuacuaucuGGCUGCAg -3' miRNA: 3'- -UCGGUg-UACUa--UGGGUUUG----------CCGACGU- -5' |
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| 9521 | 3' | -52.9 | NC_002532.2 | + | 3010 | 0.68 | 0.267284 |
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Target: 5'- aAGgCGC-UGAUGgCCAGGCGGUgGCAg -3' miRNA: 3'- -UCgGUGuACUAUgGGUUUGCCGaCGU- -5' |
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| 9521 | 3' | -52.9 | NC_002532.2 | + | 1790 | 0.69 | 0.237346 |
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Target: 5'- uGCgACguGUGGUACCCuu-UGGCUGUAa -3' miRNA: 3'- uCGgUG--UACUAUGGGuuuGCCGACGU- -5' |
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| 9521 | 3' | -52.9 | NC_002532.2 | + | 10289 | 0.72 | 0.139998 |
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Target: 5'- cGGCCACAUGGgucgUGCCUAcaGugGGCcaguUGCAc -3' miRNA: 3'- -UCGGUGUACU----AUGGGU--UugCCG----ACGU- -5' |
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| 9521 | 3' | -52.9 | NC_002532.2 | + | 11016 | 1.09 | 0.000176 |
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Target: 5'- cAGCCACAUGAUACCCAAACGGCUGCAg -3' miRNA: 3'- -UCGGUGUACUAUGGGUUUGCCGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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