miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
9523 3' -49.4 NC_002532.2 + 494 0.67 0.546711
Target:  5'- cCUUGaGC-UGUUGCAACACccugcuuucgccCAGCUGCg -3'
miRNA:   3'- -GAAC-CGuAUAGUGUUGUGc-----------GUCGACG- -5'
9523 3' -49.4 NC_002532.2 + 4398 0.68 0.463531
Target:  5'- --cGGUAUgcagcAUCACcauGCugGCuGCUGCc -3'
miRNA:   3'- gaaCCGUA-----UAGUGu--UGugCGuCGACG- -5'
9523 3' -49.4 NC_002532.2 + 1432 0.68 0.463531
Target:  5'- --cGGCGcuggUACGGgGCGUGGCUGCc -3'
miRNA:   3'- gaaCCGUaua-GUGUUgUGCGUCGACG- -5'
9523 3' -49.4 NC_002532.2 + 568 0.68 0.440326
Target:  5'- -aUGGCuaaccuagcAUCAaccACACGCAGCUGg -3'
miRNA:   3'- gaACCGua-------UAGUgu-UGUGCGUCGACg -5'
9523 3' -49.4 NC_002532.2 + 11467 0.7 0.374914
Target:  5'- --gGGCAUGUCAuCAAacaGCGCA-CUGUa -3'
miRNA:   3'- gaaCCGUAUAGU-GUUg--UGCGUcGACG- -5'
9523 3' -49.4 NC_002532.2 + 906 1.14 0.00021
Target:  5'- gCUUGGCAUAUCACAACACGCAGCUGCa -3'
miRNA:   3'- -GAACCGUAUAGUGUUGUGCGUCGACG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.